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This protocol describes how to design and build a centrifuge force microscope that is 3D printed to fit within an existing benchtop centrifuge and enables the simultaneous probing of hundreds to thousands of single-molecule interactions using tethered particles.
We present a protocol for preparing microcrystal samples for cryo-EM diffraction imaging, including room temperature solid-state small molecules and soluble and membrane protein crystals.
YCharOS is a consensus platform for antibody characterization developed through collaboration between academia and industry, enabling direct comparisons among antibodies that target a specific protein in three common applications.
Programmable addition via site-specific targeting elements (PASTE) combines the specificity, efficiency and cargo size benefits of site-specific integrases with the programmability of prime editing for precise and efficient integration of large DNA sequences into mammalian genomes.
A neuroimaging protocol presenting versatile high-resolution and time-efficient MRI acquisition and processing strategies suitable for large-scale and long-term population studies, which include structural, diffusion-weighted and functional MRI.
This deep learning platform enables large chemical spaces to be mined to identify substructures underlying predicted activity, with explainable reasoning behind the predictions. The protocol focuses on discovering structural classes of antibiotics but can be generally applied to other small molecules.
In this protocol, the tissue clearing process is carried out using a multiwell plate and insert, which allows the clearing steps to be conducted in parallel across multiple samples.
Generation and long-term culture of a human pluripotent stem cell-derived human cerebellar organoid model, which successfully replicates the cellular diversity of the fetal cerebellum along with some of its distinct cytoarchitectural features.
ChromEMT combines a fluorescent DNA binding dye that selectively enhances DNA and nucleosomes in electron microscopy with multi-tilt tomography, to enable the imaging and reconstruction of nuclear chromatin ultrastructure and 3D organization.
The facile synthesis of the 432 helicoid III nanoparticle morphology, displaying a Kuhn’s dissymmetry factor (g-factor) of 0.2, is detailed by using l-glutathione in an aqueous solution.
This protocol describes the optimization of RNA preparation conditions for cryo-EM structure determination, along with cryo-EM processing pipelines to resolve RNA dynamics and conformational changes, and workflows to generate moderate- to high-resolution cryo-EM density maps.
This protocol presents a method for generating mesoporous superparticles from monomicelle units, with precise control of the number of the monomicelle units and the derived mesopores for superparticles.
This protocol outlines a medium-throughput strategy based on combinatorial dye staining of pools of effector T cells to screen in parallel the reactivity of up to hundreds of T cell receptors against patient primary tissues or panels of antigens.
This protocol presents a method for the preparation and culture of mouse and human primary or pluripotent stem cell-derived basal cells, followed by their intra-airway transplantation into polidocanol-conditioned mice to achieve in vivo airway regeneration.
This protocol describes a bio-orthogonal method for dynamically altering the adhesiveness or stiffness of the synthetic extracellular matrix during three-dimensional culture in a spatiotemporal manner to induce phenotypic changes and to produce functional tissues.
This protocol repurposes an Illumina NovaSeq 6000 S4 flow cell for low-cost, submicrometer-resolution spatial transcriptome profiling of a tissue section and provides a comprehensive computational analysis pipeline.
A protocol for common variants of outcome devaluation tasks facilitates standardization of testing goal-directed behavior in rats by detailing control conditions, outcome pre-exposure, habituation to pre-feeding chambers and hunger levels in rats.
This targeted mass spectrometry protocol uses stable isotope-labeled peptides as internal standards for accurate, sensitive identification and quantification of peptides bound to MHCs, nominating potential targets for vaccines and immunotherapies.
