Agricultural Biotechnology articles

Time frame: 1 May 2025 - 30 April 2026

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Count: 598

Article ‘Count’ for Agricultural Biotechnology.

Journal Count
6
Artificial Intelligence-Integrated Overcoupled Resonator for Multifunctional Pesticide Spectral Classification and Quantification
A Thermogenetic Tool Employing Elastin-like Polypeptides for Controlling Programmed Cell Death
Disrupting Biofilm Tolerance by Ionic Microbubble-Mediated Copper Ion Surge for Infection Clearance
Split DNA Tetrahedron-Mediated Spatiotemporal-Hierarchy CRISPR Cascade Integrated with Au@Pt Nanolabels and Artificial Intelligence for a Cervical Cancer MicroRNA Bioassay
Functional Delivery Systems for Targeting Tumor Heterogeneity: Reversing Immunosuppression and Modulating Cancer-Immune Interplay
Overcoming Photothermal Resistance of Gastric Cancer by Bionic 2D Iron-Based Nanoplatforms with Precise CRISPR/Cas9 Delivery
1
Effective delivery of CRISPR/dCas9-SAM for multiplex gene activation based on mesoporous silica nanoparticles for bladder cancer therapy
9
From Algal Threat to Fe/N-Rich Single-Atom Catalyst: Engineering Iron-Accumulating Algae for High-Performance Fenton-Like Chemistry and Lifecycle Sustainability
Light‐Carbon Double Transfer Membrane for Carbon Fixation in Microalgae
Spatially Separated Dual‐Probiotic Hydrogel Enables Synergistic Infection Control and Tissue Healing
Nanopuncturing‐Enabled Efficient Gene Editing for Isogenic Human iPSC‐Derived Organoid Engineering
Engineered Microalgae‐Material Biohybrid Enhances Carbon‐Negative Hydrogen Production Under Emerging Contaminant Stress
Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells
Dynamic Real-Time Monitoring of Microalgal Photosynthesis Using Droplet Triboelectric Nanogenerators
Oligohistidine‐Functionalized Single‐Walled Carbon Nanotube‐Guided RNA Delivery to Improve Shoot Regeneration Efficiency in Plant Calli
An Integrated Sequential‐Activated DNA Circuit for Reliable Imaging of Intracellular miRNA and Remote Control of Cellular Function
3
Poking Pluripotency: Nanoinjection Into Human iPSCs
Accelerated CRISPR/Cas12a-Based Point-of-Care Diagnostics Through Critical Coupling Distance Control
Janus Nanohybrids Enable Superflash Warming and High‐Affinity Ice Confinement for Cross‐Scale Cryopreservation
2
Functional classification of platelet gene variants using CRISPR HDR in CD34+ cell-derived megakaryocytes
Identifying active and inhibitor-resistant MGMT variants for gene therapy
43
A Programmable Y-Shaped Catalytic Hairpin Assembly Amplification Enabling Ultrasensitive Fluorescent Analysis of Circular RNAs in Living Cells
CRISPR/dCas9-Assisted On-Bead Multiplex Detection (BeadPlex2) for Genetically Modified Crops
Analytical Assessment of sgRNA Impurities and Their Impact on Functional Performance
Enhanced SNV Detection of HLA-B*15:02TA by Integrating Blocking RPA and Inhibiting CRISPR-Cas12a
Conformationally Regulated CRISPR/Cas12a Activation Enabled by a Programmable DNA Dumbbell for Electrochemical SNP Genotyping
Stem-Engineered Aptamers with Enhanced Stability and Affinity for Tumor-Targeted Imaging
Antibody-Free and Rapid Profiling of Transcription Factor Binding Sites by Aptamer-Guided Chromatin Cleavage and Sequencing (AptaChC-Seq)
EXAGO: An Argonaute-Based Primer-Free Exponential Amplification Strategy for Ultrasensitive Zero-Background Detection of Point Mutation
Quantitative Approach for Simultaneous In Situ Profiling of Lignin, Cellulose, and Hemicellulose Using Confocal Raman Microscopy
Identification of Recombinant Adeno-Associated Virus Serotypes by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection
Integration of Demethylation-Switchable Deoxyribozyme with Controllable Self-Assembly of Branched DNA Nanostructures Enables In Situ Monitoring of FTO Demethylation in Living Cells
An Asymmetric Stem-Loop Translator Enhances Specificity of One-Pot Isothermal CRISPR/Cas12a Assay
Deoxyribonucleic Acid Looped-Ag Nanocluster-Based Fluorescent Sensor for Accurate and Sensitive Circular Ribonucleic Acid Analysis via Dual Catalytically Deactivated Cas13a/crRNA Recognition-Mediated Proximity Ligation
Single-Tube Dual-Gene Detection of Methicillin-Resistant Staphylococcus aureus via Selective Trans-Cleavage Preferences of Cas9 and Cas12a
DNA Flap-Controlled CRISPR/Cas12a Trans-Cleavage Enables Mix-and-Read FEN1 Activity Detection
Plug-and-Play Photo-Initiated CRISPR–Cas12a One-Pot Nucleic Acid Detection via Universal Repeat RNA Acylation Strategy
A Universal Light-Activated CRISPR-RNA Based on Split Direct Repeat for One-Pot Cas12a Nucleic Acid Detection
Specificity of Arsenic Stress Detection by Raman Spectroscopy During Co-Occurrences of Nitrogen Deficiency and Narrow Brown Leaf Spot
Proximity-Inducible CRISPR/Cas12a Activity by Scaffold RNA Assembly for Sensing Applications
Reaction Kinetics of CRISPR trans-Cleavage Controlled Using Isotachophoresis
Affinity-Driven Two-Step Immobilization of Endogenous Natural GABAA Receptor to Quantify Drug–Protein Analysis with Enhanced Sensitivity and Accuracy
Guanine-Quadruplex-Engineered crRNA Enables Light-Activated CRISPR/Cas12a System for Robust One-Pot Viral Assay
Stimulated Raman Scattering (SRS) Microscopy Enables Visualization and Quantification of Cryoprotective Agent Concentration in Daphnia magna
Breaking the PAM Restriction: A Universal Double Stranded DNA Detection Method Based on the Sticky End-Mediated CRISPR/Cas12a Coupled RPA and Its Application to KRAS G12C Single Base Mutations
A Chimeric Photo-Controllable CRISPR/Cas12a System for Universal and Fast Diagnostics
Fabricating Four-Element Doped Carbon Dots-Based Fluorescent Ratiometric Reporter Platform for CRISPR/Cas-Driven Precise Sensing of Nucleic Acids
Metal-Tannic Acid Nanospheres Empowering DNA Nanoarchitectures for Multimodal Detection of Low-Abundance Pesticide-Resistance Genes
CRISPR/Cas13a trans-Cleavage-Driven Programmable Autonomous Synthesis of a Functional G-Quadruplex for Ultrasensitive Detection of Circular RNAs with an Improved Signal-to-Background Ratio
Discrimination of Microalgal Cellular Metabolic Heterogeneity and sup13/supC Metabolic Flux Analysis via Micro Label-Free Mass Cytometry
5′-End Engineering of CRISPR/Cas12a Activators: A Versatile Platform for Multiple Biomarker Analysis and Clinical Cancer Tissue Identification
Lanthanide Metal–Organic Framework-Integrated CRISPR-Cas Technology for Amplification-free Gene Mutation Assay
CONVERT: Dynamic crRNA Reconfiguration for Universal One-Pot CRISPR/Cas12a-Based Nucleic Acid Detection
Split crRNA-Mediated Regulation of CRISPR-Cas12a Enables Construction of Biochemical Circuits for Sensitive Molecular Diagnostics
Combining the NanaPPI Toolbox and AI-Driven Virtual Inhibitor Screening for the p53-MDM2 Interaction
Breakthrough in LbCas12a Chemical Inhibitor Screening: The Firefly Probe and Its Application in Dual-Mode Biosensors
SPARC: An Orthogonal Cas12a/Cas13a Dual-Channel CRISPR Platform for Reliable SNV Identification and Mutation Confirmation
Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics
Reducing the Concentration of Hairpins to Weaken Their Nonspecific Adsorption on Cas12a for a Seven-Order-of-Magnitude Reduction in the Limit of Detection
Spherical Nucleic Acids as Modulators of CRISPR/Cas12a by a Steric Barrier Effect for Designing Versatile Biosensors
iThermus thermophilus/i Argonaute-Mediated Single Particle Counting Platform for Multiplex Cancer-Related Biomarkers Detection
A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies
Tri-Mode CRISPR-Based Biosensor for miRNA Detection: Enhancing Clinical Diagnostics with Cross-Validation
12
Direct Selection of Functional De Novo Macrocycles for Activation of On-Cellulo Insulin Receptor
Small-Molecule Activation of mRNA Translation by Click-to-Release Reaction in Cells
Expanded Gene Targeting in RNA Hacking With G-Tract-Supply Staple Oligomers
DNA Flap‐Mediated Control of Transcription for Programmable RNA Synthesis
Near‐Infrared Light‐Activated Circular Oligonucleotides for Spatiotemporal Control of Gene Silencing in Mice
Engineered Thermostable Chemically Responsive GlowCas9 System for Real-Time Therapeutic Monitoring Applications
Bioinformatics‐Driven Design of Peptides for Membrane Stabilization During Cryopreservation
Allosteric Activation of Cas12a via PAM Topological Engineering for Direct and Rapid Detection of Nucleases
Imaging‐Based High‐Content Screening with Clickable Probes Identifies XPB Inhibitors
Light‐Triggered CRISPR/Cas12a for Genomic Editing and Tumor Regression
Functionally Tunable Star‐Shaped Multivalent crRNAs for Photocontrol CRISPR/Cas Editing
Nuclear‐Targeted Material Enabled Intranuclear MicroRNA Imaging for Tracking Gene Editing Process
1
Advanced technology and applications of small-scale, modular, and distributed biomass direct air capture systems
1
Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency
3
Lactobacillus johnsonii Synthesizes Chenodeoxycholic Acid to Reduce Susceptibility to High-Fat Diet–Induced Colorectal Cancer
Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells
Immunological Tolerance to Luciferase and Fluorescent Proteins Using Tol Mice Enables Development of Improved Tumor Models for Investigating Immunity and Metastasis
11
Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression
StealTHY: An immunogen-free CRISPR platform to expose concealed metastasis regulators in immunocompetent models
Functional RNA splitting drove the evolutionary emergence of type V CRISPR-Cas systems from transposons
Conversion of IscB and Cas9 into RNA-guided RNA editors
AI-generated MLH1 small binder improves prime editing efficiency
Iterative recombinase technologies for efficient and precise genome engineering across kilobase to megabase scales
Identification and application of cell-type-specific enhancers for the macaque brain
Machine-learning-assisted universal protein activation in living mice
Advancing protein evolution with inverse folding models integrating structural and evolutionary constraints
Scalable generation and functional classification of genetic variants in inborn errors of immunity to accelerate clinical diagnosis and treatment
Unraveling mitochondrial influence on mammalian pluripotency via enforced mitophagy
2
Crosstalk between three CRISPR-Cas types enables primed type VI-A adaptation in Listeria seeligeri
Type I CRISPR-Cas immunity primes type III spacer acquisition
1
ChemPerturb-seq screen identifies a small molecule cocktail enhancing human beta cell survival after subcutaneous transplantation
12
A novel directed evolution platform for engineering chemically gated protein switches
Sequence-independent optical regulation of CRISPR/Cas editing using star-shaped crRNA dendrimers
Engineering guidelines for CRISPR diagnostics
A label-free orthogonal dual-channel CRISPR-Cas platform for simultaneous detection of Mycobacterium tuberculosis and respiratory syncytial virus
Harnessing conserved G-quadruplexes for fluorescence detection of Mycobacterium tuberculosis
A sticky end-driven PAM-free RPA-CRISPR/Cas12a dual amplification system for ultrasensitive detection of KRAS G12C
Integration of CRISPR/Cas12a and Cas13a in one pot for ratiometric calibration of single-nucleotide variations
CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224
Enzyme inhibition-enabled CRISPR/Cas12a biosensing system for heparin-related non-nucleic acid biomarkers
Monoethanolamine and microalgae collaboration enhances carbon sequestration and accumulation of high-value eicosapentaenoic acid
A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation
A novel CRISPR–Cas12a-based fluorescence anisotropy method with a high signal-to-background ratio for sensitive biosensing
9
A suite of macrocyclic peptide inhibitors and substrate probes for arginine methyltransferases
cShot: spatial dynamic imaging of cell cycle-dependent miRNA heterogeneity using dynamic DNA patterns
Spatiotemporally regulated mitochondrial genome editing via enzyme and NIR-activated CRISPR/Cas9 nanoplatform
Reprogramming chemically induced dimerization systems with genetically encoded nanobodies
Turn-engineering tunes the conformational rigidity of β-hairpin AMPs in achieving membrane selectivity and killing drug-resistant ESKAPE pathogens
Light-controlled genome editing by activation of Cas9-mRNA translation
THRUST: translesion synthesis-driven hierarchical regulation using a template-activator construct for Cas12a activity
Regulating CRISPR/Cas12a trans-cleavage on the hairpin DNA–MB nanointerface for enhanced multiplexed sensing application
Multi-STEM MePCR: a bisulfite-free, multiplex, highly sensitive and highly specific assay to measure DNA methylation
4
Mitigating xenogeneic barriers to chimerism through Cas13-induced host attenuation
Single-cell activation screen identifies hepatic maturation regulators with zonal resolution
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology
zHORSE as an optogenetic zebrafish strain for precise spatiotemporal control over gene expression during development
6
Prediction of nitrogen product distribution in algal pyrolysis bio-oil driven by machine learning
Synergistic coupling of microalgae and corn cob residue biochar for enhanced lipid production and heavy metal removal from waste leachate and corn cob hydrolysate
Multiphysics Synergy Analysis of Light, CO2 Mass Transfer and Fluid Dynamics in Microalgae Photobioreactors
Comparative techno-economic and life cycle assessment of microalgae-based CO2 capture technologies for coal-fired power plants under different carbon supply modes
Transforming sludge-containing urban wastewater to clean energy and biochemicals via an algae-based carbon-neutral pyrolytic pathway
New insights into efficient lipid production and decolorization of Congo red by microalgae under anaerobic environment: Performance and degradation mechanisms
3
Dynamic extracellular polymeric substance component succession primes cyanobacterial metabolism for distinct bloom stages
Tolerance strategy of Chlorella sorokiniana to combined antibiotic stress revealed by transcriptomic analysis
Chroococcus minor cathode and polyphenolic mediated anode for enhancing bioenergy and bioremediation in a novel microbial desalination cell
13
Removal of trace 17α-ethinylestradiol by microalgal-bacterial granular sludge: The overlooked role of extracellular photosensitization
Resource utilization and microbial community evolution in anaerobic digestion effluent containing heavy metal Zn via eco-remediation technology based on a PBR algal-bacterial symbiosis system
Microalgae-fungi co-cultivation for swine wastewater treatment: Insights into EPS-mediated aggregation mechanism
Unveiling the algicidal effects and compounds of a newly isolated algicidal Enterococcus sp. JL7
Phosphate-Iron Modified Enteromorpha Prolifera Hydrochar Enhances Dry Anaerobic Digestion of Food Waste: Synergistic Mechanisms of Electron Transfer Network, Microbial Consortia Remodeling, and Metagenomic Insights
Synergistic modulation of sludge hydrolysate-borne bacteria on glycogen accumulation and carbon fixation mechanisms in microalgae
Innovative and high efficiency harvesting process of Chlorella vulgaris by magnetic harvesting
Nitrate modulates high CO2-alters carbon partitioning in Gracilariopsis lemaneiformis: Trade-offs between particulate and dissolved organic carbon sequestration
New Insights into Fungi-Mediated Flocculation of Chlorella vulgaris: A Transcriptomics - Metabolomics Integration from Biosynthesis to Mechanism
Development and performance evaluation of a circulating fixed-bed photobioreactor (CFBP) with clinoptilolite fillers for enhanced microalgae cultivation in urine: mechanistic insights
Rapid and efficient biosorption of Pb(II) by high-nitrate cultivated microalgae
Algae Biofilm Produces Less Microbe-Derived Dissolved Organic Nitrogen Under Higher C/N Ratio Conditions
Comparative analysis of species-specific dissolved gaseous mercury oxidation in phytoplankton cultures
8
The Bioplastic Library of Polymers (BLoP): A Raman and μATR-FTIR Spectroscopy Library for Detection of Bioplastics
Photosystem-Driven Resilience of Green Microalga Tetraselmis chuii toward Acute Nonylphenol Stress
Mismatch-Driven CRISPR/Cas12a Biosensing of UV-Induced DNA Lesions for Environmental Solar Exposure Surveillance
Genetic Markers Remain Detectable in Genetically Engineered Microbes Biocontained with a CRISPR Kill Switch
Revealing Single-Cell Heterogeneity of Labile Cu(I) Accumulation and Metabolism in Microalgae by Image-Enabled Flow Cytometry
Microbially Driven Organic Carbon Degradation and Nutrient Cycling during Macroalgal Decomposition
Redox Control of Copper Biotransformation and Its Toxicity Cascades in Microalgae
Biodegradation of Arsenosugars from Red Alga Pyropia haitanensis
1
Gastric Organoid-Based Ectopic and Orthotopic In Vivo CRISPR Screening for Tumor Suppressors in Gastric Cancer
4
Highly efficient and scarless genome editing via essential gene-coupled homology-directed repair
High-resolution genotype-free mapping of genetic variation with CRI-SPA-Map
Restoring the potency of neutralizing antibody via guided hypermutation with hyper-antibody editor
3′-end ligation sequencing is a sensitive method to detect DNA nicks at potential sites of off-target activity induced by prime editors
1
Nearshore Macroalgae Cultivation for Carbon Sequestration by Biomass Harvesting: Evaluating Potential and Impacts With an Earth System Model
8
A photoactivatable Cre-loxP system for spatiotemporal genetic manipulation in mouse taste buds
A genetic manipulation tool based on the GP35 recombinase for targeted gene editing in mycoplasmas of ruminants
Genome-wide CRISPR interference screen identifies Clip2 as a novel regulator of osteocyte maturation and morphology
Mismatch type impacts interference and priming activities in the type I–E CRISPR–Cas system
PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators
The complete λ-carrageenan depolymerization cascade from a marine Pseudoalteromonad revealed by structural analysis of the enzymes
Branched DNA processing by a thermostable CAS-Cas4 from Thermococcus onnurineus: Expanding biochemical landscape of nuclease activity
In vivo pulse-chase in Caenorhabditis elegans reveals intestinal histone turnover changes upon starvation
2
Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
Cell type–specific spatiotemporal control of GFP-tagged protein degradation in mice
17
Prediction and optimization of light transmission to enhance microalgae growth in built-in light sources photobioreactor for efficient CO2 fixation
Fe-doped quantum dots boost synergistic dual-cycle carbon fixation for value-added products synthesis in anoxygenic photoheterotrophs: an innovative strategy toward high-efficiency wastewater valorization with net carbon reduction
Development and application of a CRISPR/Cas9n system for deleting multiple secretion-related protein genes in Bacillus amyloliquefaciens to facilitate industrial enzymes clean production
Process design and techno-economic evaluation of hydrogen production from microalgae conversion in molten salts
Assessment of Rugulopteryx okamurae seaweed as source of sustainable alginate gels with polyphenols from orange peel
Coordinating sulfamethoxazole removal and fouling control through species–ratio configurations in membrane microalgal–bacterial coupling systems
Utilizing food waste hydrolysate for enhanced microalgae protein production: a step towards circular economy
Techno-economic framework for sustainable use of microalgae for industrial production – A bioplastics case study
Screening and characterization of marine cyanobacteria for dairy farm wastewater treatment: Nodosilinea sp. E11 as a promising candidate for phycoremediation
Efficient biosorption of molybdenum (VI) using Chlorella pyrenoidosa biomass: Mechanistic insights and sustainable application in water remediation
Two-stage cultivation using noodle soup waste water for the sustainable production of microalgal biomass and biodiesel
Mechanisms of naphthalene biodegradation in microalgal-bacterial co-culture systems: role of photosynthesis, antioxidant and extracellular polymeric substances
Efficient amplification of marine dark carbon fixation at the bioreactor scale: Bottlenecks and regulating strategies
Model system for recovering nitrogen and phosphorus from yellow water to supply a microalgae culture on a building facade
Production of renewable fuels and platform chemicals from carbon dioxide using microalgae: Economic and environmental perspective
Phototrophic microalgae-driven comprehensive remediation of arsenic and cadmium: A novel iron mineralization pathway enabling arsenic immobilization
Techno-economic analysis of Spirulina biomass production in the floating photobioreactors for fishmeal replacements
2
Gene therapy via CRISPR/Cas9-mediated Cxcr4 disease allele inactivation reverses leukopenia in WHIM mice
Single-dose genome editing therapy rescues auditory and vestibular functions in adult mice with DFNA41 deafness
1
A iCandidozyma/i (iCandida/i) iauris–/iOptimized Episomal Plasmid–Induced Cas9-Editing System Reveals the Direct Impact of the S639F-Encoding iFKS1/i Mutation
1
Aberrant medial prefrontal cortex activity and flexible behavior in the TgF344-AD rat model of Alzheimer's disease
7
Conditional Localization Pharmacology Manipulates the Cell Cycle with Spatiotemporal Precision
Streamlined Synthesis and Immunomodulatory Effects of Ulvan Oligosaccharides from Marine Green Algae
A Computationally Optimized Ribonucleic Acid Circularization Strategy without Byproducts
Creating a Modular Activatable CRISPR–Cas12a System by Engineering crRNA Scaffold with a Steric Hindrance Effector
Photocontrolled Programmable Enzymatic Cascade for Robust CRISPR Diagnostics
A Molecular Glue Approach to Control the Half-Life of CRISPR-Based Technologies
Synthetic CRISPR Networks Driven by Transcription Factors via Structure-Switching DNA Translators
10
Rapid customization of base editors via machine learning-powered combinatorial mutagenesis
The discovery of 5mC-selective deaminases and their application to ultra-sensitive direct sequencing of methylated sites at base resolution
Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system
CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samples
Structural insights into Cas9-mediated prespacer selection in CRISPR-Cas adaptation
Structural basis for Cas9-directed spacer acquisition in type II-A CRISPR-Cas systems
miRNA modules for precise, tunable control of gene expression
Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaks
Engineered hypercompact Fanzor-ωRNA system with enhanced genome editing activity
Structural insights into DdCBE in action enable high-precision mitochondrial DNA editing
2
Lifestyle shapes preclinical social and microglial deficits in an Alzheimer’s disease mouse model
Chronic social isolation-unpredictable stress induces early-onset cognitive deficits and exacerbates Aβ accumulation in the 5xFAD mouse model of Alzheimer’s disease
5
Near-Infrared Light-Inducible CRISPR-Cas12a/Tetrahedral DNA Nanosystem for Spatiotemporal Control of mtDNA Mutation Imaging and Gene Therapy
A Nanoimprinted Photothermal Chip for On-Demand Spatiotemporal Activation of CRISPR/Cas9 Gene Editing
PBAE-PEG/Lipid Nanoparticle Delivery of RNA for the Creation of Genetically Engineered Lung Cancer Mouse Models
Simply Engineered crRNA with CRISPR/Cas12a System Enables Wide-Scope Nucleic Acid Biomarker Analysis
Tetraspanin 4 Mediates Cholesterol-Dependent Exosome Membrane Protection from Cryodamage
23
Prime assembly with linear DNA donors enables large genomic insertions
Quadruple pegRNA enables programmable and efficient large genomic insertion
Molecular basis for methylation-sensitive editing by Cas9
DNA damage drives antigen diversification in Trypanosoma brucei
Synthetic super-enhancers enable precision viral immunotherapy
Structural basis of supercoiling-induced CRISPR–Cas9 off-target activity
In vivo site-specific engineering to reprogram T cells
Synthetic circuits for cell ratio control
Immune evasive DNA donors and recombinases license kilobase-scale writing
In vivo base editing of Chd3 rescues behavioural abnormalities in mice
Scalable and multiplexed recorders of gene regulation dynamics across weeks
Ultra-high-throughput mapping of genetic design space
RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity
Gene-drive-capable mosquitoes suppress patient-derived malaria in Tanzania
Semantic design of functional de novo genes from a genomic language model
Prime editing-installed suppressor tRNAs for disease-agnostic genome editing
Connecting chemical and protein sequence space to predict biocatalytic reactions
Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
Engineered prime editors with minimal genomic errors
Design of highly functional genome editors by modelling CRISPR–Cas sequences
Precisely defining disease variant effects in CRISPR-edited single cells
Rewiring endogenous genes in CAR T cells for tumour-restricted payload delivery
Programmable control of spatial transcriptome in live cells and neurons
33
Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism
Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering
Diversity-generating retroelements for programmable targeted hypermutagenesis
Inducible, split base editors for in vivo cancer functional genomics
Sequence Display enables large-scale sequence–activity datasets for rapid protein evolution
Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression
Precise, minimally evolved adenine base editors generated through mutation reversion analysis
Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning
Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system
Trimodal single-cell profiling of transcriptome, epigenome and 3D genome in complex tissues with scHiCAR
Single-round evolution of RNA aptamers with GRAPE-LM
Customizing CRISPR–Cas PAM specificity with protein language models
CRISPR–Cas3-based editing for targeted deletions in a mouse model of transthyretin amyloidosis
Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE
Single-strand deaminase-assisted editing for functional RNA manipulation
Engineered base editors with reduced bystander editing through directed evolution
Multiplexed profiling of transcriptional regulators in plant cells
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording
Site-specific DNA insertion into the human genome with engineered recombinases
CRISPR live-cell imaging reveals chromatin dynamics and enhancer interactions at multiple non-repetitive loci
Discovery and engineering of retrons for precise genome editing
Integrated epigenetic and genetic programming of primary human T cells
Programmable promoter editing for precise control of transgene expression
Discovery and protein language model-guided design of hyperactive transposases
Design of optimized epigenetic regulators for durable gene silencing with application to PCSK9 in nonhuman primates
Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes
Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase
Efficient mitochondrial A-to-G base editors for the generation of mitochondrial disease models
A mitochondrial disease model is generated and corrected using engineered base editors in rat zygotes
Dual SORT LNPs for multi-organ base editing
A multi-kingdom genetic barcoding system for precise clone isolation
Evolution-guided protein design of IscB for persistent epigenome editing in vivo
8
Genetic recording and in situ readout of single-cell signaling memory
Modular engineering of thermoresponsive allosteric proteins
Abasic CRISPR RNAs inherently harness fidelity of SpCas9 for genome editing
De novo design of potent CRISPR–Cas13 inhibitors
A gasdermin-based life–death evolution system for reprogramming protease specificity
Efficient and precise inversion of genomic DNA from large to chromosomal scale
Repurposing salicylic acid as a versatile inducer of proximity
Engineering eukaryotic transposon-encoded Fanzor2 system for genome editing in mammals
1
Photocatalytic labelling-enabled subcellular-resolved RNA profiling and synchronous multi-omics investigation
165
Technical and biological sources of noise confound multiplexed enhancer AAV screening
Faecalibacterium prausnitzii EXL01 for the Maintenance of Steroid-induced Clinical Response or Remission in Patients with Crohn’s Disease: a first in human trial
Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency
A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation
Molecular and physiological acclimation to low light and iron scarcity in a globally abundant oceanic pelagophyte
Photoactivatable CRISPR/Cas13d via upconversion nanoparticles for deep tissue RNA engineering and orthopedic therapy
Sensitive monitoring of enhancer and noncoding RNA transcription via ribozyme-assisted RNA editing
Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation
Genome-wide CRISPR screens in primary human natural killer cells identify countermeasures against immunosuppressive environment
Efficient genome editing with chimeric oligonucleotide-directed editing
Sex distorter male drive for resistance-resilient population control of the human malaria vector Anopheles gambiae
Mode of action guided metagenomic natural product discovery reveals convergent evolution of a ClpP-targeting motif
Activation mechanism and integrated one-pot assay of chiral-like cRNA-enhanced CRISPR/Cas12a systems
Molecular basis of target RNA cleavage by Cas13
Skeletal editing via multi-step engineering of a modular polyketide synthase
Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation
Structural insights into C-terminus-mediated RNA target cleavage by a mesophilic prokaryotic argonaute
Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapy
Engineered microbial hydrogels with confined architecture and binary microbes for efficient hydrogen production
AcrIIA7 hijacks tracrRNA to block CRISPR-Cas system
Integrated computational and experimental immunoengineering of adeno-associated virus capsid T cell epitopes in mice
Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing
Target-stabilized base editors enable robust high-fidelity RNA editing
ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks
CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization
Vulnerability of short-term memory in a mouse model of Alzheimer’s disease
Engineered Un1Cas12f1 for multiplex genome editing with enhanced activity and targeting scope
H pilin cyclisation and pilus biogenesis are promiscuous but electrostatic perturbations impair conjugation efficiency
Causes and consequences of experimental variation in transient expression
dHyperCas12a enables multiplexed CRISPRi screens
Uncovering Cas9 PAM diversity through metagenomic mining and machine learning
Decoding efficacy and resistance space at a drug binding site
Engineered bacterial therapy suppresses Enterohemorrhagic through metabolic competition and virulence silencing
Engineering plasmids with synthetic origins of replication
Bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation
Click-based determination of accumulation of molecules in Escherichia coli
CRISPR-Cas9 trans-cleavage is hindered by a flanked R-loop, an elongated spacer, and an inactive HNH domain
Breaking the cell wall for efficient DNA delivery to diatoms
Engineering the MmeFz2-ωRNA system for efficient genome editing through an integrated computational-experimental framework
Af-CUTTag: a sensitive and antibody-free chromatin profiling method using genetically encoded tags and high-affinity binders fused to Tn5
Coiled-coil heterodimer-mediated split base editing systems enable flexible and robust nucleotide substitutions
Identification of thermotolerant non-canonical PAMs for robust one-pot CRISPR-Cas12a detection
Improved in vivo gene knockout with high specificity using multiplexed Cas12a sgRNAs
Microbial activation of the GLP-2R mitigates gastrointestinal inflammation
Single-cell multiplex approaches deeply map ON-target CRISPR-genotoxicity and reveal its mitigation by palbociclib and long-term engraftment
A compact and inducible dCas12f-based CRISPRa platform for programmable in vivo gene activation
In vivo chemogenetic RNA editing of macrophages by bioengineered viruses for sepsis treatment
Interactions with bacteria shape diatom adaptation to carbon concentration changes
Remediation and upcycling of microplastics by algae with wastewater nutrient removal and bioproduction potential
Computational design of allulose-responsive biosensor toolbox for auto-inducible protein expression and CRISPRi mediated dynamic metabolic regulation
A Plug-in system for reprogramming the editing patterns of base editors
Manganese-engineered Lactobacillus Reuteri with enhanced antitumor and immunomodulatory activities for colorectal cancer prevention and treatment
Anti-CRISPR protein AcrIIA5 can enhance the activity and security of prime editing
A pooled Cell Painting CRISPR screening platform enables de novo inference of gene function by self-supervised deep learning
Protein-nucleic acid language model-assisted design of precise and compact adenine base editor
Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida
Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation
Engineering the auxin-inducible degron system for tunable in vivo control of organismal physiology
Mosquito sex separation using complementation of selectable traits and engineered neo-sex chromosomes
Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication
Editing DNA methylation in vivo
Efficient high-precision transgene knock-in by Recombinases (Redα/β)-enhanced DNA integration-CRISPR-Cas9 (RED-CRISPR)
Ligase-mediated programmable genomic integration (L-PGI)
Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation
Personalized CRISPR knock-in cytokine gene therapy to remodel the tumor microenvironment and enhance CAR T cell therapy in solid tumors
The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contexts
Search-and-remove genome editing allows selection of cells by DNA sequence
Architecture and mechanism of a dual-enzyme retron system in prokaryotic immunity
CRISPR-free RNA base editing mediated PTC-readthrough restores hearing in mice with nonsense mutation
A next-generation dual guide CRISPR system for genetic interaction library screening
Versatile molecular tools enabling customizable DNA methylation editing in Arabidopsis
A flanking-nicks prime editor (FLICK-PE) system to boost prime editing in dicots
Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity
Programmable targeted RNA degradation via dCas13d-directed chaperone-mediated autophagy (dCasCMA)
Reduced Cas9 transgene silencing by incorporation of intron sequences
Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos
Continuous targeted hypermutation with a tunable mutation window
Amphibious microneedles for programmable delivery of biomolecules and microorganisms in living plants
A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release
A yeast surface display platform for characterizing CAR T cell responses to cancer antigens
Combinatorial protein engineering identifies potent CRISPR activators with reduced toxicity
Circular single stranded DNA potentiates non-viral gene insertion in hematopoietic stem and progenitor cells
Characterizing and controlling CRISPR repair outcomes in nondividing human cells
Deep generative optimization of mRNA codon sequences for enhanced mRNA translation and therapeutic efficacy
Deep learning models simultaneously trained on multiple datasets improve base-editing activity prediction
Effective in vivo RNA base editing via engineered cytidine deaminase APOBECs fused with PUF proteins
Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR
A temperature-sensitive CRISPR-Cas12a system for sterile insect technique
CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis
Reprogramming encapsulins into modular carbon-fixing nanocompartments
Noncanonical target-strand cytosine base editing via engineered Un1Cas12f1 platform
Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-making
Structure-function relationship of the GH168 fucanase reveals an unusual enzyme recognition mechanism for sulfated polysaccharide
A motif preferred adenine base editor with minimal bystander and off-targets editing
HLA matching or CRISPR editing of HLA class I/II enables engraftment and effective function of allogeneic human regulatory T cell therapy in a humanized mouse transplantation model
Tailoring Cas12a functionality with a user-friendly and versatile crRNA variant toolbox
citrOgen: a synthesis-free polysaccharide and protein antigen-presentation to antibody-induction platform
Cassini: streamlined and scalable method for in situ profiling of RNA and protein
Genetic controllers for enhancing the evolutionary longevity of synthetic gene circuits in bacteria
De novo design of hypercompact transcript degraders by engineering substrate-specific toxins and Cas6-CBS system
Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation
A streamlined base editor engineering strategy to reduce bystander editing
Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins
Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells
Discovery of CRISPR-Cas12a clades using a large language model
Dual-key cooperatively activated DNA regulator for controlling mitochondria-lysosome interactions
Targeted CRISPR screens reveal genes essential for survival in the host intestine
Matrix regulation: a plug-and-tune method for combinatorial regulation in
Diminished immune cell adhesion in hypoimmune ICAM-1 knockout human pluripotent stem cells
Targeted gene editing and near-universal cDNA insertion of CYBA and CYBB as a treatment for chronic granulomatous disease
Structural insights into Type II-D Cas9 and its robust cleavage activity
Self-splicing RNA circularization facilitated by intact group I and II introns
Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes
Computational identification of small molecules for increased gene expression by synthetic circuits in mammalian cells
GFP-on mouse model for interrogation of in vivo gene editing
Direct high-throughput deconvolution of non-canonical bases via nanopore sequencing and bootstrapped learning
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox
Identification of a key nucleotide influencing Cas12a crRNA activity for universal photo-controlled CRISPR diagnostics
Amino acid transporters mediate autonomous delivery of nanoparticle vehicles into living plants
Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technology
The PAZ pocket and dimerization drive CpAgo’s guide-independent and DNA-guided dual catalysis
Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes
Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC)
Refined DNA repair manipulation enables a universal knock-in strategy in mouse embryos
Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout
Regulating cleavage activity and enabling microRNA detection with split sgRNA in Cas12b
Random access and semantic search in DNA data storage enabled by Cas9 and machine-guided design
Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons
Cas12a cleavage mediated lateral flow assay enables multiplex and ultra-specific nucleic acid detection
Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA
Personalized azithromycin treatment rules for children with watery diarrhea using machine learning
An orthogonal transcription mutation system generating all transition mutations for accelerated protein evolution in vivo
Enhancing biolistic plant transformation and genome editing with a flow guiding barrel
Benchmarking metabolic RNA labeling techniques for high-throughput single-cell RNA sequencing
Mechanisms and engineering of a miniature type V-N CRISPR-Cas12 effector enzyme
Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo
The virulence regulator CovR boosts CRISPR-Cas9 immunity in Group B
Visualization of a multi-turnover Cas9 after product release
Deep mutational scanning identifies Cas1 and Cas2 variants that enhance type II-A CRISPR-Cas spacer acquisition
Engineered Sdd7 cytosine base editors with enhanced specificity
Mice with a diverse human T cell receptor repertoire selected on multiple HLA class I molecules
The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2
Somatic gene delivery faithfully recapitulates a molecular spectrum of high-risk sarcomas
Mechanism of DNA degradation by CBASS Cap5 endonuclease immune effector
Prime editor with rational design and AI-driven optimization for reverse editing window and enhanced fidelity
Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell death
Precision multiplexed base editing in human cells using Cas12a-derived base editors
Circular RNA-mediated inverse prime editing in human cells
Spatially blocked split CRISPR-Cas12a system for ultra-sensitive and versatile small molecule activation and detection
hpCasMINI: An engineered hypercompact CRISPR-Cas12f system with boosted gene editing activity
Multiplex base editing to protect from CD33 directed drugs for immune and gene therapy
An engineered U7 small nuclear RNA scaffold greatly increases ADAR-mediated programmable RNA base editing
Reactive oxygen species responsive nanomotors for gene edited metabolic disruption and immunotherapy
Robust prediction of synthetic gRNA activity and cryptic DNA repair by disentangling cellular CRISPR cleavage outcomes
The latent cis-regulatory potential of mobile DNA in Escherichia coli
Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screens
A generalizable approach for programming protease-responsive conformationally inhibited artificial transcriptional factors
Engineering tripartite gene editing machinery for highly efficient non-viral targeted genome integration
Comprehensive synthesis and anticoagulant evaluation of a diverse fucoidan library
Large-scale discovery of potent, compact and erythroid specific enhancers for gene therapy vectors
Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems
A nuclear-encoded endonuclease governs the paternal transmission of mitochondria in Cucumis plants
Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder
Construction of multi-targeted CRISPR libraries in tomato to overcome functional redundancy at genome-scale level
2
Activity-based selection for enhanced base editor mutational scanning
Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states
5
CREsted: modeling genomic and synthetic cell-type-specific enhancers across tissues and species
A portable poison exon for small-molecule control of mammalian gene expression
Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase
De novo assembly and delivery of synthetic megabase-scale human DNA into mouse early embryos
ATLAS: a rationally designed anterograde transsynaptic tracer
2
In vivo CRISPR screen reveals regulation of macrophage states in neuroinflammation
CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes
6
Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency
Structural insight into IscB’s RNA-lid-based inactivation mechanism
Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions
Structural visualization of the molecular evolution of CRISPR–Cas9
An anti-CRISPR targets the sgRNA to block Cas9 and guides the design of enhanced genome editors
Computational design of a high-precision mitochondrial DNA cytosine base editor
2
An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes
Enhancer AAV toolbox for accessing and perturbing striatal cell types and circuits
1
Indopexines A and B: Two Prenylated Isoindoline Alkaloids from the Fungus Aspergillus sp. TJ507
3
CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin–antitoxin activity when horizontally transferred
Overexpression of the pioneer transcription factor Nr5a2 promotes the development of mouse somatic cell nuclear transfer embryos
Correction of pathogenic mitochondrial DNA in patient-derived disease models using mitochondrial base editors
4
Development of an RNA aptamer-assisted CRISPR/Cas9 system for efficiently generating and isolating Cas9-free mutants in plant
High-throughput conjugation reveals strain specific recombination patterns enabling precise trait mapping in Escherichia coli
Performance of two low-threshold population replacement gene drives in cage populations of the yellow fever mosquito, Aedes aegypti
DNA duplication in Burkholderia thailandensis induces biofilm formation by activating a two-component regulatory system.
36
Field Trials and Baking Studies of Ultra-Low Asparagine, Genome Edited (CRISPR/Cas9) and Mutant (TILLING) Wheat
Enhancing CRISPR/Cas-Mediated Gene Knockout With Short Non-Homologous Oligonucleotides
<atl>Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in iNicotiana benthamiana/i</atl>
Engineering Marker‐Free Lettuce Chloroplast Genome to Express Functional Glucagon‐Like Peptide‐1 Receptor Agonists Exenatide and Lixisenatide
Cas9-Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A-To-G Base Editing in Rice
Protein-Derived Signal Peptides Induced by Agrobacterium Infection Promote the Secretion of Recombinant Proteins in Nicotiana benthamiana
Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses
TKC-MC: An Effective Strategy for Generating Heritable Heterozygous Mutations in Essential Genes in Rice
Transgene-Free, Gene-Edited Cavendish Bananas (Musa acuminata, AAA)
Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants
Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR–Cas9 Mediated TcNPR3 Mutagenesis
Versatile Applications of CRISPR-Based Programmable T-DNA Integration in Plants
CRISPR/Cas9‐Mediated Knockouts of the iALG3/i and iGNTI/i in iN. benthamiana/i and Their Application to Pharmaceutical Production
Heterologous Production of Cyprosin B in Nicotiana benthamiana: Unveiling the Role of the Plant-Specific Insert Domain in Protein Function and Subcellular Localisation
Computationally Guided Design of a Soluble, Abundant and Functional AnfH Variant for the Expression of Fe-Only Nitrogenase in Plant Mitochondria
Red Alga Porphyridium Supports High-Yield Production of a Functional Chimeric Hepatitis B Surface Antigen With Strong Cellular and Humoral Immunogenicity
Transgene-Killer-CRISPR version 2 (TKC2) eliminates occasional transgene escape by coupling with a RUBY reporter
Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.)
Bioengineered poplar fibres via PagGLR2.8 editing: A synergistic design for high-performance biocomposites
Rice-derived SARS-CoV-2 glycoprotein S1 subunit vaccine elicits humoral and cellular immune responses
Seamless, modular binary vectors for plant cell and molecular biology
Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato
A visual monitoring DNA-free multi-gene editing system excised via LoxP::FRT/FLP in poplar
Morphology effect of a novel biocompatible nucleic acid delivery nanosystem of g-C3N4@dsRNA for application in plant gene expression and plant virus disease protection
Highly efficient transgene-free ErCas12a RNP-protoplast genome editing and single-cell regeneration in Nicotiana benthamiana for glyco-engineering
Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus
Cas9- and Cas12a-mediated excision and replacement of the celiac disease-related α-gliadin immunogenic complex in hexaploid wheat
Expression of a constitutively active nitrate reductase increases SARS‐CoV‐2 Spike protein production in iNicotiana benthamiana/i leaves that otherwise show traits of senescence
Highly efficient Agrobacterium rhizogenes-mediated gene editing system in Salvia miltiorrhiza inbred line bh2-7
Efficient clonal seeds sorting for apomictic hybrid rice using a pollen‐specific gene switch system
Cas9-Rep fusion tethers donor DNA in vivo and boosts the efficiency of HDR-mediated genome editing
Non-destructive seed genotyping via microneedle-based DNA extraction
Direct and indirect effects of multiplex genome editing of scpF5H/scp and scpFAD2/scp in oil crop camelina
Harnessing novel cytidine deaminases from the animal kingdom for robust multiplexed base editing in rice
Harnessing haploid‐inducer mediated genome editing for accelerated maize variety development
Expression of endochitinase and exochitinase in lettuce chloroplasts increases plant biomass and kills fungal pathogen Candida albicans
26
Improving photosynthesis by scattering hydrogel fiber–enabled volumetric illumination
A modified CRISPR/Cas9 approach in silencing the triplication in Down syndrome: A treatment path XISTs
An acetyltransferase family required for biosynthesis of diverse marine carotenoids
Molecular kinetics dictate population dynamics in CRISPR-based plasmid defense
Cellular antibody affinity–based CRISPR screening identifies JUNB as a broadly acting antiviral factor
Functional recovery of the adult murine hippocampus after cryopreservation by vitrification
Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas
Towards CRISPR-based editing of the mitochondrial genome in yeast
Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes
Chlamydomonas chloroplast genes tolerate compression of the genetic code to just 51 codons
Acclimation to high and low diurnal light is flexible in Chlamydomonas reinhardtii
An ADAR2-mimic base editor for efficient C-to-U RNA editing in vivo
A chemical epigenetic tool to probe site-specific DNA-binding protein complexes
Versatile Xenopus tropicalis model with targeted integration of human BRAFV600E
A general genome editing strategy using CRISPR lipid nanoparticle spherical nucleic acids
TIGER: A tdTomato in vivo genome-editing reporter mouse for investigating precision-editor delivery approaches
STAGE: A compact and versatile TnpB-based genome editing toolkit for Streptomyces
Targeted deletions of large syntenic regions in Arabidopsis thaliana
Hide-and-Seek genome editing reveals that Gephyrin is required for axo-axonic synapse assembly
Engineered Un1Cas12f1 with boosted gene-editing activity and expanded genomic coverage
Protective antigen–mediated delivery of an anti-CRISPR protein for precision genome editing
Synthesis of large single-transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter
In vivo generation of CAR macrophages via the enucleated mesenchymal stem cell delivery system for glioblastoma therapy
Mycobacteriophage-mediated gene transfer enables in vitro drug screening and in vivo tracking of Mycobacterium leprae
High-throughput metabolic engineering of Yarrowia lipolytica through gene expression tuning
Quadruple adenine base–edited allogeneic CAR T cells outperform CRISPR/Cas9 nuclease–engineered T cells
9
Gene syntax defines supercoiling-mediated transcriptional feedback
Programmable genome editing in human cells using RNA-guided bridge recombinases
Multistep genomics on single cells and live cultures in subnanoliter capsules
CRISPR-Cas–mediated heritable chromosome fusions in Arabidopsis
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases
Engineered geminivirus replicons enable rapid in planta directed evolution
A comprehensive genetic catalog of human double-strand break repair
Megabase-scale human genome rearrangement with programmable bridge recombinases
Programmable gene insertion in human cells with a laboratory-evolved CRISPR-associated transposase
20
CRISPRi-based functional genomic screening identifies genes essential for CH4-dependent growth in a methanotrophic bacterium
Protecting cells at the genetic level and simulating unauthorized access via a biohackathon
Mitochondrial heterogeneity drives the evolution of fungicide resistance in Phytophthora sojae, with associated fitness trade-offs
A programmable genetic platform for engineering noninvasive biosensors
Identification of proteins influencing CRISPR-associated transposases for enhanced genome editing
Targeting transthyretin by one Cas9 variant with superfidelity and broad compatibility
Targeted lung Lactobacillus johnsonii intervention alleviates virus-induced fibrosis post-HCT through PD-L1/PD-1 signaling
Programmable multistep CRISPR gene activation via control of RNA polymerase III termination
Renal clearable CRISPR nanosensor targeting mitochondrial DNA mutation for noninvasive monitoring of tumor progression and metastasis
Oligo-CALL: A next-generation barcoding platform for studying resistance to targeted therapy
The editable landscape of the yeast genome reveals hotspots of structural variant formation
Antibiotic stress–induced hormesis in phytoplankton and bacteria through their mutual cooperation
CRISPR/Cas9-germline editing of Biomphalaria glabrata: A breakthrough in genetic modification of snails that transmit schistosomiasis
AI-directed gene fusing prolongs the evolutionary half-life of synthetic gene circuits
BioFuse: A programmable timer switch of gene expression
Integrating incompatible tandem photobiocatalysis in artificial cells enables metabolic modulation of natural cells
A machine-learning–powered spectral-dominant multimodal soft wearable system for long-term and early-stage diagnosis of plant stresses
Structures of vertebrate R2 retrotransposon complexes during target-primed reverse transcription and after second-strand nicking
Upgraded circular single-stranded DNA regulators for multiple-input multiple-output gene circuits in mammalian cells
Multiplexing light-inducible recombinases to control cell fate, Boolean logic, and cell patterning in mammalian cells
1
Base editing of β0-thalassemia mutations as a therapeutic strategy for severe β-hemoglobinopathies
2
Assessing target genes for homing suppression gene drive
A bacterial gene acquired by parasitoid wasps contributes to venom secretion against host defence
5
Cellular membrane protein MipA from E.coli Nissle 1917 protects against Salmonella infection
Reactive oxygen species trigger downward vertical migration in diatom microphytobenthic biofilms as a strategy to cope with oxidative stress
Temperature-dependent photostasis and nitrogen limitation in streamlined-genome red algae Cyanidiophyceae from natural habitats
Lotka-Volterra Dynamics Facilitate Sustainable Biocontrol of Wastewater Sludge Bulking
iEubacterium limosum/i modulates tumor microenvironments and produces antitumor metabolites active against colorectal cancer
3
Investigation of Methionine Metabolism in Coccolithophore by iIn Situ/i Light-Coupled Nuclear Magnetic Resonance Spectroscopy
Kinetic Mechanism for Fidelity of CRISPR-Cas9 Variants
dTAT1: An Unnatural Nucleoside Exhibiting Low Photocytotoxicity for Genetic Code Expansion
1
Home administration of a multistrain probiotic once per day for 4 weeks to newborn infants in Tanzania (ProRIDE): a double-blind, placebo-controlled randomised trial
2
Identification of a cleaved aberrant RNA associated with the initiation of transgene silencing
Too dim, too bright, and just right: Systems analysis of the iChlamydomonas/i diurnal program under limiting and excess light
27
Extracellular polymeric substances trigger microalgae flocculation in a combined and green flocculation-flotation harvesting process
Illumination-Induced Restructuring of Extracellular Polymeric Substances from Klebsiella Sp. HDJY-1 Drives Indirect Photodegradation of Thifensulfuron-methyl
Mechanistic responses of coccolithophores to exposure to the marine pollutant atrazine
Unlocking microbial synergy in microalgae-bacteria granules: RSM-driven optimization and lifecycle microbial metagenomics for high-ammonia wastewater treatment
Effect of Nitrosomonas europaea on Chlorella vulgaris in bio-hydrogels for startup of microalgal-bacterial granular sludge: Performance and microscopic mechanism
Water lifting aerators control algal growth in drinking water reservoirs: Performance, mechanism and application
Stabilizing photogranule systems under low-light and limited-aeration: From operational strategy to underlying mechanism
Biomass Ratio Regulates Methane Conversion and Carbon Fixation in a Methanotrophs-Microalgae Symbiotic System: Efficiency Optimization and Mechanisms Driven by Co-metabolism
Legacy effect of repeated stimulation by Indole-3-Acetic Acid on the rapid attachment of microalgae biofilms
How Chlorella vulgaris remove florfenicol from wastewater—— from the aspects of kinetics, mechanism, limiting factors and long-term regulation
Coupling Microalgae-based Biochar with MBGS Enhances Microbial Synergy and Multi-Pollutant Removal from Saline Aquaculture Wastewater
Photocatalysis-fueled algae-bacteria mutualism enables P450 enzyme-induced nitrite-free anammox for sustainable nitrogen removal
Comparative study of CO₂ nanobubbles and macrobubbles: Effects on water chemistry, microalgal growth, and carbon utilization
Sticky or thrifty: Divergence in extracellular glycometabolic architecture underpins contrasting fungal bioflocculation strategies
Crosstalk with Chlorella sp. reshapes the environmental fates of aspartame: From stress responses to contaminant destabilization
Projected area calculation for microalgae using three-dimensional models
Community succession and protein enhancement in a mixed methanotroph-microalgae system with stepwise increase of ammonium loading - Inhibition and adaptation
Bifunctional fungi trade off denitrification and algicidal performance to inhibit algal growth and control algal bloom: Denitrification-algicidal interactions, organic matter dynamics, and raw water treatment
Using bio-based CaCO3 functionalized sediment to simultaneously remove algae and COD through adsorption and sedimentation in water source reservoirs
Microalgae-mediated treatment of steel industry reverse osmosis concentrate integrated with flue gas carbon fixation: From laboratory selection to pilot-scale verification
Hormetic effect of dissolved organic matter from pig manure anaerobic digestion effluents on Chlorella sp.: Physiological and transcriptomic responses
Synergistic microalgal-bacterial interactions enhance nitrogen removal in membrane-aerated biofilm photoreactors treating aquaculture wastewater under salt stress: Insights from metagenomic analysis
Semi-continuously operated single strain microalgal-bacterial biofilm for nutrients removal: microbial succession and performance improvement over extended non-axenic operation
Layer-specific photo-metabolic specialization of encapsulated microalgae: A strategy for synchronous multi-nitrogen elimination from wastewater
Reduction of Antibiotics and Antibiotic Resistance Genes in Simulated-Sunlight-Supported Counter-diffusion Bacteria-Algae Biofilms: Interface Properties and Functional Gene Responses
CO2 Drives Ecological Shifts through Asymmetric Selection in Microalgae-Bacteria Granular for Enhanced Wastewater Treatment and Carbon Fixation
Morphotype-Resolved Characterization of Microalgal Communities in a Nutrient Recovery Process with ARTiMiS Flow Imaging Microscopy

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