Agricultural Biotechnology articles for United States of America (USA)

Time frame: 1 June 2025 - 30 May 2026

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Count: 231

Article ‘Count’ for Agricultural Biotechnology.

Journal Count Share
2 0.98
Nanopuncturing‐Enabled Efficient Gene Editing for Isogenic Human iPSC‐Derived Organoid Engineering 0.08
Versatile Cell Penetrating Peptide for Multimodal CRISPR Gene Editing in Primary Stem Cells 0.90
2 2.00
Functional classification of platelet gene variants using CRISPR HDR in CD34+ cell-derived megakaryocytes 1.00
Identifying active and inhibitor-resistant MGMT variants for gene therapy 1.00
7 4.72
Analytical Assessment of sgRNA Impurities and Their Impact on Functional Performance 1.00
Single-Tube Dual-Gene Detection of Methicillin-Resistant Staphylococcus aureus via Selective Trans-Cleavage Preferences of Cas9 and Cas12a 0.14
Specificity of Arsenic Stress Detection by Raman Spectroscopy During Co-Occurrences of Nitrogen Deficiency and Narrow Brown Leaf Spot 1.00
Reaction Kinetics of CRISPR trans-Cleavage Controlled Using Isotachophoresis 0.90
Stimulated Raman Scattering (SRS) Microscopy Enables Visualization and Quantification of Cryoprotective Agent Concentration in Daphnia magna 1.00
Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics 0.50
A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies 0.18
1 0.98
Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency 0.98
1 0.04
Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells 0.04
6 4.05
Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression 0.07
Tuning the sensitivity of mechanosensory receptors through histidine scanning 0.03
Human genetics guides the discovery of CARD9 inhibitors with anti-inflammatory activity 1.00
Conversion of IscB and Cas9 into RNA-guided RNA editors 1.00
Scalable generation and functional classification of genetic variants in inborn errors of immunity to accelerate clinical diagnosis and treatment 1.00
Unraveling mitochondrial influence on mammalian pluripotency via enforced mitophagy 0.96
1 1.00
Crosstalk between three CRISPR-Cas types enables primed type VI-A adaptation in Listeria seeligeri 1.00
4 1.87
Genotoxicity profiling reveals distinct platform-and cell type-specific effects in therapeutic gene editing for genetic hyperinflammation 0.13
Minimizing far-extending chromatin perturbation in genome editing preserves stem cell identity 0.05
Prevention of transgene silencing during human pluripotent stem cell differentiation 0.69
ChemPerturb-seq screen identifies a small molecule cocktail enhancing human beta cell survival after subcutaneous transplantation 1.00
2 1.75
A novel directed evolution platform for engineering chemically gated protein switches 1.00
Engineering guidelines for CRISPR diagnostics 0.75
1 1.00
Reprogramming chemically induced dimerization systems with genetically encoded nanobodies 1.00
2 1.73
Mitigating xenogeneic barriers to chimerism through Cas13-induced host attenuation 0.73
A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biology 1.00
1 1.00
Genetic Markers Remain Detectable in Genetically Engineered Microbes Biocontained with a CRISPR Kill Switch 1.00
2 2.00
High-resolution genotype-free mapping of genetic variation with CRI-SPA-Map 1.00
3′-end ligation sequencing is a sensitive method to detect DNA nicks at potential sites of off-target activity induced by prime editors 1.00
4 3.94
Genome-wide CRISPR interference screen identifies Clip2 as a novel regulator of osteocyte maturation and morphology 0.94
Mismatch type impacts interference and priming activities in the type I–E CRISPR–Cas system 1.00
PIONEER: A Periplasmic Display Platform for Synthetic Biology-Based Screening of Genetically Encoded Protein Regulators 1.00
In vivo pulse-chase in Caenorhabditis elegans reveals intestinal histone turnover changes upon starvation 1.00
1 1.00
Cell type–specific spatiotemporal control of GFP-tagged protein degradation in mice 1.00
3 2.93
Simultaneous CRISPR/Cas9-induced double-strand breaks are lethal in models of pancreatic cancer 1.00
Gene therapy via CRISPR/Cas9-mediated Cxcr4 disease allele inactivation reverses leukopenia in WHIM mice 1.00
Single-dose genome editing therapy rescues auditory and vestibular functions in adult mice with DFNA41 deafness 0.93
1 1.00
A iCandidozyma/i (iCandida/i) iauris–/iOptimized Episomal Plasmid–Induced Cas9-Editing System Reveals the Direct Impact of the S639F-Encoding iFKS1/i Mutation 1.00
1 1.00
Aberrant medial prefrontal cortex activity and flexible behavior in the TgF344-AD rat model of Alzheimer's disease 1.00
2 2.00
Conditional Localization Pharmacology Manipulates the Cell Cycle with Spatiotemporal Precision 1.00
A Molecular Glue Approach to Control the Half-Life of CRISPR-Based Technologies 1.00
5 3.94
Gut microbiota modulation of regulatory DNA elements revealed by massively parallel functional characterization 0.14
The discovery of 5mC-selective deaminases and their application to ultra-sensitive direct sequencing of methylated sites at base resolution 1.00
CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samples 1.00
miRNA modules for precise, tunable control of gene expression 0.80
Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaks 1.00
1 1.00
Chronic social isolation-unpredictable stress induces early-onset cognitive deficits and exacerbates Aβ accumulation in the 5xFAD mouse model of Alzheimer’s disease 1.00
1 1.00
PBAE-PEG/Lipid Nanoparticle Delivery of RNA for the Creation of Genetically Engineered Lung Cancer Mouse Models 1.00
17 13.40
RNA-triggered cell killing with CRISPR–Cas12a2 0.52
Prime assembly with linear DNA donors enables large genomic insertions 1.00
Molecular basis for methylation-sensitive editing by Cas9 0.71
DNA damage drives antigen diversification in Trypanosoma brucei 0.89
Synthetic circuits for cell ratio control 0.33
In vivo site-specific engineering to reprogram T cells 0.99
Immune evasive DNA donors and recombinases license kilobase-scale writing 1.00
Scalable and multiplexed recorders of gene regulation dynamics across weeks 0.91
Ultra-high-throughput mapping of genetic design space 1.00
RNA-triggered Cas12a3 cleaves tRNA tails to execute bacterial immunity 0.17
Prime editing-installed suppressor tRNAs for disease-agnostic genome editing 1.00
Semantic design of functional de novo genes from a genomic language model 1.00
Connecting chemical and protein sequence space to predict biocatalytic reactions 0.86
Engineered prime editors with minimal genomic errors 1.00
Design of highly functional genome editors by modelling CRISPR–Cas sequences 0.96
Precisely defining disease variant effects in CRISPR-edited single cells 1.00
Rewiring endogenous genes in CAR T cells for tumour-restricted payload delivery 0.06
26 23.07
AI-guided redesign of laboratory-evolved reverse transcriptases enhances prime editing 1.00
Directed evolution of small RNA-stabilizing motifs that improve prime-editing efficiency 1.00
DNA-guided CRISPR–Cas12 for cellular RNA targeting 1.00
A single-cell screening platform accelerates functional genetics in plants 1.00
Pool-packaged AAV libraries exhibit extensive length-dependent and homology-dependent chimerism 0.94
Genome editing of phylogenetically distinct bacteria using cross-species retron-mediated recombineering 0.79
Diversity-generating retroelements for programmable targeted hypermutagenesis 0.14
Inducible, split base editors for in vivo cancer functional genomics 1.00
Sequence Display enables large-scale sequence–activity datasets for rapid protein evolution 1.00
Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression 1.00
Precise, minimally evolved adenine base editors generated through mutation reversion analysis 1.00
Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning 1.00
Trimodal single-cell profiling of transcriptome, epigenome and 3D genome in complex tissues with scHiCAR 0.87
Customizing CRISPR–Cas PAM specificity with protein language models 1.00
Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity using CHANGE-seq-BE 1.00
Engineered base editors with reduced bystander editing through directed evolution 0.95
Multiplexed profiling of transcriptional regulators in plant cells 1.00
A parts list of promoters and gRNA scaffolds for mammalian genome engineering and molecular recording 1.00
Site-specific DNA insertion into the human genome with engineered recombinases 1.00
Discovery and engineering of retrons for precise genome editing 1.00
Integrated epigenetic and genetic programming of primary human T cells 0.96
Programmable promoter editing for precise control of transgene expression 1.00
Targeted DNA ADP-ribosylation triggers templated repair in bacteria and base mutagenesis in eukaryotes 0.35
Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates 0.08
High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase 1.00
Dual SORT LNPs for multi-organ base editing 1.00
2 1.94
Genetic recording and in situ readout of single-cell signaling memory 0.94
Repurposing salicylic acid as a versatile inducer of proximity 1.00
72 49.36
Tunable gene control via RNA splicing with a clinically approved small molecule 0.15
Simultaneous orthogonal cell engineering by a single CRISPR-Cas9 polyfunctional editor 0.06
Structural basis for double-stranded DNA cytosine deamination by BaDTF3 and its application in mitochondrial genome editing 0.38
Technical and biological sources of noise confound multiplexed enhancer AAV screening 1.00
A combinatorial domain screening platform reveals epigenetic effector interactions for transcriptional perturbation 1.00
Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potency 1.00
Photoactivatable CRISPR/Cas13d via upconversion nanoparticles for deep tissue RNA engineering and orthopedic therapy 0.04
Molecular and physiological acclimation to low light and iron scarcity in a globally abundant oceanic pelagophyte 0.77
Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation 0.11
Efficient genome editing with chimeric oligonucleotide-directed editing 1.00
Mode of action guided metagenomic natural product discovery reveals convergent evolution of a ClpP-targeting motif 1.00
Skeletal editing via multi-step engineering of a modular polyketide synthase 0.08
Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapy 0.05
Integrated computational and experimental immunoengineering of adeno-associated virus capsid T cell epitopes in mice 1.00
Nucleotide-resolution mapping of regulatory elements via allelic readout of tiled base editing 0.60
CRAGE-RB-PI-seq reveals transcriptional dynamics of plant-associated bacteria during root colonization 0.98
H pilin cyclisation and pilus biogenesis are promiscuous but electrostatic perturbations impair conjugation efficiency 0.13
Causes and consequences of experimental variation in transient expression 1.00
dHyperCas12a enables multiplexed CRISPRi screens 1.00
Engineering plasmids with synthetic origins of replication 1.00
Bridge helix of Cas12a is an allosteric regulator of R-loop formation and RuvC activation 1.00
Click-based determination of accumulation of molecules in Escherichia coli 0.86
Remediation and upcycling of microplastics by algae with wastewater nutrient removal and bioproduction potential 1.00
A pooled Cell Painting CRISPR screening platform enables de novo inference of gene function by self-supervised deep learning 1.00
Automation and machine learning drive rapid optimization of isoprenol production in Pseudomonas putida 1.00
Protein-nucleic acid language model-assisted design of precise and compact adenine base editor 0.09
Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation 1.00
Editing DNA methylation in vivo 1.00
Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication 1.00
Ligase-mediated programmable genomic integration (L-PGI) 1.00
Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation 0.12
Personalized CRISPR knock-in cytokine gene therapy to remodel the tumor microenvironment and enhance CAR T cell therapy in solid tumors 0.02
The Endo-GeneScreen platform identifies drug-like probes that regulate endogenous protein levels within physiological contexts 1.00
Argonaute-HNH filaments triggered by invader DNA confer bacterial immunity 0.25
Reduced Cas9 transgene silencing by incorporation of intron sequences 0.93
A yeast surface display platform for characterizing CAR T cell responses to cancer antigens 0.05
Combinatorial protein engineering identifies potent CRISPR activators with reduced toxicity 0.94
Characterizing and controlling CRISPR repair outcomes in nondividing human cells 1.00
Effective in vivo RNA base editing via engineered cytidine deaminase APOBECs fused with PUF proteins 0.18
A temperature-sensitive CRISPR-Cas12a system for sterile insect technique 0.80
Unveiling the cut-and-repair cycle of designer nucleases in human stem and T cells via CLEAR-time dPCR 0.13
CRISPR targeting of H3K4me3 activates gene expression and unlocks centromere-proximal crossover recombination in Arabidopsis 0.18
Engineering wetware and software for the predictive design of compressed genetic circuits for higher-state decision-making 1.00
A motif preferred adenine base editor with minimal bystander and off-targets editing 0.13
HLA matching or CRISPR editing of HLA class I/II enables engraftment and effective function of allogeneic human regulatory T cell therapy in a humanized mouse transplantation model 0.04
Cassini: streamlined and scalable method for in situ profiling of RNA and protein 1.00
Physical vitrification and nanowarming at liter-scale CPA volumes: toward organ cryopreservation 1.00
A streamlined base editor engineering strategy to reduce bystander editing 1.00
Cytotoxicity of activator expression in CRISPR-based transcriptional activation systems 1.00
Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins 0.96
Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells 0.80
Targeted CRISPR screens reveal genes essential for survival in the host intestine 0.10
Diminished immune cell adhesion in hypoimmune ICAM-1 knockout human pluripotent stem cells 1.00
Iterative SCRaMbLE for engineering synthetic genome modules and chromosomes 0.07
GFP-on mouse model for interrogation of in vivo gene editing 1.00
Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolbox 1.00
Systematic comparison and base-editing-mediated directed protein evolution and functional screening yield superior auxin-inducible degron technology 1.00
Efficient, cell-type-specific production of flavonols by multiplexed CRISPR activation of a suite of metabolic enzymes 0.33
Higher resolution pooled genome-wide CRISPR knockout screening in Drosophila cells using integration and anti-CRISPR (IntAC) 1.00
Deep-tissue high-sensitivity multimodal imaging and optogenetic manipulation enabled by biliverdin reductase knockout 0.96
Random access and semantic search in DNA data storage enabled by Cas9 and machine-guided design 1.00
Structure and biochemistry-guided engineering of an all-RNA system for DNA insertion with R2 retrotransposons 1.00
Deep mutational scanning identifies Cas1 and Cas2 variants that enhance type II-A CRISPR-Cas spacer acquisition 1.00
Personalized azithromycin treatment rules for children with watery diarrhea using machine learning 0.55
Visualization of a multi-turnover Cas9 after product release 1.00
Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo 0.95
Enhancing biolistic plant transformation and genome editing with a flow guiding barrel 1.00
Mice with a diverse human T cell receptor repertoire selected on multiple HLA class I molecules 0.50
The green algae CO2 concentrating mechanism and photorespiration jointly operate during acclimation to low CO2 0.06
Somatic gene delivery faithfully recapitulates a molecular spectrum of high-risk sarcomas 0.03
Mechanism of DNA degradation by CBASS Cap5 endonuclease immune effector 1.00
Selective targeting of genome amplifications and repeat elements by CRISPR-Cas9 nickases to promote cancer cell death 1.00
2 2.00
Activity-based selection for enhanced base editor mutational scanning 1.00
Comprehensive transcription factor perturbations recapitulate fibroblast transcriptional states 1.00
4 3.07
Multiplexed perturbation enables scalable pooled screens 1.00
CREsted: modeling genomic and synthetic cell-type-specific enhancers across tissues and species 0.07
A portable poison exon for small-molecule control of mammalian gene expression 1.00
Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase 1.00
1 1.00
CRISPR screening by AAV episome-sequencing (CrAAVe-seq): a scalable cell-type-specific in vivo platform uncovers neuronal essential genes 1.00
2 1.27
Comparative characterization of Cas12f orthologs reveals mechanistic features underlying enhanced genome editing efficiency 1.00
Structural visualization of the molecular evolution of CRISPR–Cas9 0.27
1 0.17
CRISPR-Cas is beneficial in plasmid competition, but limited by competitor toxin–antitoxin activity when horizontally transferred 0.17
1 1.00
Performance of two low-threshold population replacement gene drives in cage populations of the yellow fever mosquito, Aedes aegypti 1.00
9 8.33
Engineering Marker‐Free Lettuce Chloroplast Genome to Express Functional Glucagon‐Like Peptide‐1 Receptor Agonists Exenatide and Lixisenatide 1.00
Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses 1.00
Development of Modular Geminivirus-Based Vectors for High Cargo Expression and Gene Targeting in Plants 1.00
Reduced Susceptibility to Phytophthora in Non-Transgenic Cacao Progeny Through CRISPR–Cas9 Mediated TcNPR3 Mutagenesis 1.00
Viral-mediated delivery of morphogenic regulators enables leaf transformation in Sorghum bicolor (L.) 1.00
Seamless, modular binary vectors for plant cell and molecular biology 1.00
Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus 1.00
Cas9- and Cas12a-mediated excision and replacement of the celiac disease-related α-gliadin immunogenic complex in hexaploid wheat 0.33
Non-destructive seed genotyping via microneedle-based DNA extraction 1.00
18 17.96
Tuning mitotic recombination with patterned DNA nicks for precision mosaic analysis 1.00
Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells 1.00
Amplification-free dual-blocking autocatalytic CRISPR-Cascade for attomolar DNA detection with low nonspecific signal 1.00
Improving photosynthesis by scattering hydrogel fiber–enabled volumetric illumination 1.00
A modified CRISPR/Cas9 approach in silencing the triplication in Down syndrome: A treatment path XISTs 1.00
Cellular antibody affinity–based CRISPR screening identifies JUNB as a broadly acting antiviral factor 1.00
Metabolic rewiring and biomass redistribution enable optimized mixotrophic growth in Chlamydomonas 1.00
Towards CRISPR-based editing of the mitochondrial genome in yeast 1.00
Rewinding evolution in planta: A Rubisco-null platform validates high-performance ancestral enzymes 1.00
Acclimation to high and low diurnal light is flexible in Chlamydomonas reinhardtii 1.00
A chemical epigenetic tool to probe site-specific DNA-binding protein complexes 1.00
A general genome editing strategy using CRISPR lipid nanoparticle spherical nucleic acids 1.00
TIGER: A tdTomato in vivo genome-editing reporter mouse for investigating precision-editor delivery approaches 1.00
Targeted deletions of large syntenic regions in Arabidopsis thaliana 1.00
Hide-and-Seek genome editing reveals that Gephyrin is required for axo-axonic synapse assembly 1.00
Protective antigen–mediated delivery of an anti-CRISPR protein for precision genome editing 1.00
Synthesis of large single-transcript pathways from oligonucleotide pools: Design of STARBURST, an autobioluminescent reporter 0.96
Mycobacteriophage-mediated gene transfer enables in vitro drug screening and in vivo tracking of Mycobacterium leprae 1.00
4 3.51
Gene syntax defines supercoiling-mediated transcriptional feedback 0.67
Multistep genomics on single cells and live cultures in subnanoliter capsules 1.00
Metagenomic editing of commensal bacteria in vivo using CRISPR-associated transposases 1.00
Megabase-scale human genome rearrangement with programmable bridge recombinases 0.85
12 9.58
PlasmidGPT: A generative framework for plasmid analysis and generation 0.13
The genetic landscape of antibiotic sensitivity in Staphylococcus aureus 1.00
CRISPRi-based functional genomic screening identifies genes essential for CH4-dependent growth in a methanotrophic bacterium 1.00
Protecting cells at the genetic level and simulating unauthorized access via a biohackathon 1.00
A programmable genetic platform for engineering noninvasive biosensors 1.00
Targeted lung Lactobacillus johnsonii intervention alleviates virus-induced fibrosis post-HCT through PD-L1/PD-1 signaling 1.00
Identification of proteins influencing CRISPR-associated transposases for enhanced genome editing 1.00
Programmable multistep CRISPR gene activation via control of RNA polymerase III termination 1.00
Oligo-CALL: A next-generation barcoding platform for studying resistance to targeted therapy 1.00
The editable landscape of the yeast genome reveals hotspots of structural variant formation 0.42
CRISPR/Cas9-germline editing of Biomphalaria glabrata: A breakthrough in genetic modification of snails that transmit schistosomiasis 0.25
Structures of vertebrate R2 retrotransposon complexes during target-primed reverse transcription and after second-strand nicking 0.79
1 0.13
RNA-LNP–mediated in vivo prime editing corrects disease phenotypes in a mouse model of citrullinemia type I 0.13
1 0.07
A bacterial gene acquired by parasitoid wasps contributes to venom secretion against host defence 0.07
1 1.00
Kinetic Mechanism for Fidelity of CRISPR-Cas9 Variants 1.00
2 2.00
Identification of a cleaved aberrant RNA associated with the initiation of transgene silencing 1.00
Too dim, too bright, and just right: Systems analysis of the iChlamydomonas/i diurnal program under limiting and excess light 1.00
4 1.13
Unlocking microbial synergy in microalgae-bacteria granules: RSM-driven optimization and lifecycle microbial metagenomics for high-ammonia wastewater treatment 0.09
Comparative study of CO₂ nanobubbles and macrobubbles: Effects on water chemistry, microalgal growth, and carbon utilization 0.83
Crosstalk with Chlorella sp. reshapes the environmental fates of aspartame: From stress responses to contaminant destabilization 0.11
Hormetic effect of dissolved organic matter from pig manure anaerobic digestion effluents on Chlorella sp.: Physiological and transcriptomic responses 0.09

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