Rhodamine derivatives with different core atoms at the 10th position exhibit diverse optical properties for fluorescence imaging, however, the effect of the 10th core atom on vibrational properties remain largely underexplored. Here, the authors prepare 9-cyanopyronin derivatives in which the 10th core atom is substituted with group 13, 14, 15 or 16 elements and evaluate their properties as resonance Raman probes, performing live-cell three-color imaging using distinct patterns of C ≡ N or C = C bond vibrations together with different subcellular probe localization.
- Shun-suke Kanai
- Hiroyoshi Fujioka
- Mako Kamiya
