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Hereditary nucleotide excision repair deficiencies cause different cancerous and progeroid disorders of which the exact etiology is not understood. This study finds that prolonged binding of DNA repair factor TFIIH to DNA damage contributes to a more severe phenotype caused by DNA repair deficiency.

The organization of membrane proteins is critical to cellular function. Here the authors explore how computational protein design, MD simulation, and cell-free systems can be combined to elucidate how membrane-protein hydrophobic mismatch affects protein folding and organization in synthetic lipid membranes.

Thermoelectric materials can convert temperature gradients into electrical potential; however, traditional materials have unfavorable mechanical, optical, and electrical properties for devices. Here, the authors design an ionic hydrogel system optimizing these properties for human-machine interface devices.

Photovoltaic devices attached to immune cells travel through the blood to inflamed brain regions.

Mutations in GJB2 cause DFNB1, the most common hereditary deafness. ATAC-seq identification of gene regulatory elements enables targeted GJB2 delivery to cochlear cells, preventing hearing loss in mouse models.

This paper highlights the far-red chemigenetic H₂O₂ reporter oROS-HT₆₃₅, which enables detailed insights into intricate intracellular and intercellular H₂O₂ dynamics, along with their environmental interactants, through spatially resolved, multiplexed real-time H₂O₂ imaging.

Enduring changes in synaptic efficacy are highly sensitive to stress. Here, the authors show that astrocytic delivery of metabolites has an important role in the stress-mediated impairment of synaptic plasticity.

Bernier, Hefendehl et al. describe a stromal progenitor cell population consisting of pericytes, venular smooth muscle cells and perivascular fibroblasts in the adult brain that modify their function and coordinate blood vessel repair following stroke.

Surgical sterilization alone cannot control global stray and feral domestic cat populations. Here, Godin et al. show that a single-dose injectable female sterilant administered before puberty safely and completely prevents pregnancy after maturity.

Yücel et al. examine how skin and hair characteristics impact functional near-infrared spectroscopy (fNIRS) signal quality and provide recommendations for inclusive use of fNIRS.

B cells need at least two signals to terminally differentiate into antibody-secreting cells. Pierce and colleagues show that persistent exposure to antigen in the absence of T cell help or ‘pathogen pattern motifs’ leads to B cell death via a calcium-dependent ‘metabolic timer’.

Yang et al. identify RHOA, a GTP hydrolyzing enzyme, as a key regulator of contact sites between mitochondria and the endoplasmic reticulum. It controls contact sites by modulating a tethering complex between the two organelles.

Different adaptive mechanisms have been reported to reduce the efficacy of mutant BRAF inhibition in melanoma. Here, the authors show BRAF inhibition induces the translational regulation of metabolic genes leading to acquired therapy resistance.

Spatial transcriptomic analysis of cells in intestinal fistulae of patients with Crohn’s disease reveals the existence of specialized fistula-associated cell states with distinct signalling profiles and extracellular matrix architecture.

Multicolor imaging employing genetically-encodable fluorescent proteins permits spatiotemporal live cell imaging of multiple cues. Here, authors use multicolor lifetime imaging to visualize quadruple-labelled human immunodeficiency viruses within cellular contexts.

In this study, the authors develop a flavivirus vaccine strategy by introducing mutations into envelope glycoproteins resulting in structural changes that conceal the ADE-prone fusion loop epitope. They show that the Zika virus-specific construct protects mice against viral challenge and prevents ADE by Dengue virus.

Phase transitions in quantum matter are related to correlation effects and they can change the ordering of material. Here the authors measure the first-order spatial correlation and the de Broglie wavelength for both thermal and condensed form of a photonic Bose gas in a dye-filled optical microcavity.

The bacterial channel protein MscL opens in response to mechanical forces and could be exploited for vesicular-based drug delivery. Doerneret al. show that functional MscL can be expressed in mammalian cells and facilitate the controlled cellular uptake of relatively large, membrane-impermeable bioactive molecules.

Konturek-Ciesla et al show that low intensity conditioning with antibody mediated haematopoietic stem cell (HSC) depletion coupled to HSC transplantation boosts blood cell and immune cell generation in aging mice, slowing disease. This approach may help treat age-related blood disorders in humans.

The Ebola virus (EBOV) glycoprotein transmembrane domain interacts with membrane cholesterol. This interaction is required for viral fusion and cell entry and may explain why statins suppress EBOV infection.

The evolution of insecticide resistance in the major malaria vector, Anopheles gambiae, remains an important issue in sustainable malaria control in Africa. Here, the authors present a framework for identifying resistance mechanisms before they arise in field mosquito populations. The findings have implications for public health surveillance and vector control.

Two-photon imaging and in situ transcriptomic analysis of the primary visual cortex in mice show that a single transcriptomic axis correlates with the state modulation of cortical inhibitory neurons.

The field of cellular agriculture has relied on 3D bioprinting for the generation of sophisticated products. Here, the authors employ chaotic bioprinting to create plant and animal cell-based hybrid noodles, thereby opening avenues to produce complex culinary designs and to explore diverse nutritional alternatives.

S protein interacts with and activates PBP1a during cell division, as part of a larger GpsB-associated multiprotein complex that coordinates peptidoglycan remodelling in Streptococcus pneumoniae.

Experiments and computations show that the organization and dynamics of molecules within and at interfaces of biomolecular condensates are governed by differential interaction strengths leading to the classification of adsorbents versus scaffolds.

TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.

Sm FRET studies of PARP-1 recognition of DNA lesion show that binding of F1F2 fragment results in a kinked DNA, while binding of F2 fragment alone yields an intermediate state. This dynamic equilibrium of states is influenced by inhibitors.

Here, the authors report that combining Army Liposome Formulation with QS21 saponin (ALFQ) and Alum to formulate the ΔV1gp120 boost decreases the risk of simian immunodeficiency virus (SIV) mac251 acquisition in female macaques.

Analysis of the longest-lived mammal, the bowhead whale, reveals an improved ability to repair DNA breaks, mediated by high levels of cold-inducible RNA-binding protein.   

Conventional blue denim dyeing has both environmental and health-related consequences. Here, Bidart et al. use enzyme engineering to develop a viable method for the bulk production of indican and demonstrate dying processes which could significantly reduce the negative consequences of this billion-dollar industry.

Xenotransplantation of a genetically edited pig kidney with a thymic autograft into a brain-dead human for 61 days with immunosuppression resulted in stable kidney function without proteinuria, and xenograft rejection was treated and reversed by the end of the study.

This study demonstrates that the extracellular matrix (ECM) of the developing brain stabilizes recently born synapses. The authors identify a microglial metalloprotease that digests the ECM to increase the plasticity of these newborn synapses.

Single molecule tracking of fluorescent proteins in live cells is temporally limited by fluorophore photobleaching. Here the authors show using fluorophore pairs that FRET competes with photobleaching to improve photostability and allow longer-term tracking of both single proteins and complexes.

The non-invasive detection and imaging of bacterial infection in living organisms is increasingly important. Here, the authors demonstrate the use of labelled carbon nanotubes as bacterial probes in living hosts, and are able to image deep tissue infections with higher signal amplification than dye imaging.

Zuhra and Petrosino et al. report evidence that cyanide acts as a regulatory gasotransmitter in mammalian cells, where it is shown to affect cellular bioenergetics, most likely via protein S-cyanylation.

The inner hair cells (IHCs) within the cochlea convey sound information and have been thought to be electrically and metabolically independent from each other. Here authors report that a subset of IHCs are electrochemically coupled in ‘mini-syncytia’.

Polyamide-12 is the main polymer employed to produce 3D objects by laser sintering constraining the functionality of the items produced. Here, the authors report a clean and scalable approach for the functionalization of polyamide-12 particles, yielding materials that can be printed using commercial apparatus.

Microdroplets are used as chemical and biological reactors; however, stability and inter-droplet transfer are major issues. Here, the authors report on the development of dendritic glycerol-based surfactants for the creation of stable microdroplets and demonstrate application for PCR, minimal emulsion, and cell encapsulation.

The authors identify defective viral particles, in people with non-suppressible HIV-1, that can replicate through superinfection and interfere with the wild-type virus. However, they show no evidence of these preventing disease progression in the individuals studied.

The development of materials for the effective removal of oils and solvents from water is of global importance. Here, the authors show that porous, hexagonal boron nitride nanosheets are capable of absorbing many times their own weight in oil while repelling water, and may be cleaned for reuse by heating or burning in air.

Creating new materials requires novel approaches to design and synthesize small building particles. Sacanna et al. develop a versatile synthetic strategy to design and mass-produce colloidal building blocks starting from two different colloids that leads to selectively functionalized surface areas.

HIV remission of more than 6 years was achieved in a patient with functional viral co-receptors after CCR5 wild-type/Δ32 allogeneic stem cell transplantation, providing evidence of other mechanisms that can be harnessed to attain long-term remission.