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CryoEM sample preparation for low abundance or preferentially oriented particles remains challenging. Jianbing Ma et al. develop the mspSA affinity-grid method that enriches particles on EM grids and reduces air-water interface exposure, demonstrating usefulness for several difficult samples.

The SARS-CoV-2 Omicron variant spreads rapidly. Here the authors show that Omicron S preferentially adopts the one-RBD-up conformation, which leads to a non-RBM-binding monoclonal antibody escape. Mutagenesis reveals that S371L, S373P and S375F substitutions enhance the conformational stability.

m336 is an exceptionally potent germline-like antibody against the emerging MERS-CoV virus. Here, the authors solve the structure of m336 in complex with MERS-CoV receptor-binding domain and use it to reveal a role of junctional and allele-specific residues in the interaction and suggest implications for vaccine development.

Miniature of electronic devices is attractive yet challenging due to structural variation at nanoscale. Here, Gao et al. report atomic imaging of reconstruction and unusual domain walls on Pb(Zr_0.2Ti_0.8)O₃surfaces, providing possibilities to engineer nanoscale structural change.

SEPAR is a computational approach that uses spatial metagenes to reveal localized gene programs, identify spatially variable genes, delineate spatial domains, and refine gene expressions for spatial transcriptomics, and uncover coordinated interactions for spatial multi-omics.

Comprehensive molecular characterisations could shed light on the high heterogeneity and poor prognosis of gliomas. Here, the authors perform proteomic profiling of 188 glioma patients, revealing immune and metabolic neuron-related subgroups as well as metabolic biomarkers linked to prognosis.

The mechanisms underlying circadian homeostasis remain elusive. Here, the authors show that anti-Müllerian hormone (Amh)-mediated signaling sustains molecular clock oscillations in the pituitary gland, thereby maintaining circadian homeostasis at both tissue and systemic levels.

The primary entry route of vanilloid ligands to the vanilloid-binding site in transient receptor potential vanilloid 1 (TRPV1) is found to be a distinct and targetable hydrophobic pathway at the TRPV1–cell membrane interface rather than through direct membrane penetration.

First-principles calculations demonstrated that the substitution of In for Ge has a lower energy barrier for phase transition than the substitution of In for Co in MnCoGe alloys. ELF calculations further reveal the regulated hysteresis’s atomic coordination origin. This theoretical prediction is completely verified by combining neutron, magnetic and calorimetric measurements; consequently, a largely enhanced barocaloric effect has been achieved.

The interfacial energy losses at the perovskite/electron selective contact interface remain an obstacle for perovskite/silicon tandem solar cells to approach its theoretical efficiency limit. Here, authors employ a cage-like diammonium chloride molecule to modulate interfacial dipole.

Gridization is useful for creating multifunctional organic semiconductors. Here, the authors report a C-H activatived gridization to synthesize UV-emitting molecular nanohydrocarbon, setting high external quantum efficiency for hydrocarbon-based UV-OLEDs.

In the multicenter phase 2 OptiTROP-Lung01 trial in patients with treatment-naive advanced non-small-cell lung cancers, the antibody–drug conjugate sacituzumab tirumotecan with anti-PD-L1 tagitanlimab was safe and showed encouraging clinical efficacy.

The evolutionarily conserved complex, the Par proteins, regulates cell polarity. Here, the authors show that in Drosophila neuroblasts, the Par complex exhibits liquid–liquid phase separation dependent on the cell cycle.

The authors investigate the impacts of drought legacy on springtime leaf unfolding and green-up. They show that drought delays springtime phenology, primarily through exogenous environmental memory effects, and suggest that future spring advances may be dampened by increasing drought.

Spatial multi-omics profiles multiple molecular modalities in the native spatial context. Here, authors introduce MultiGATE, a deep learning tool with a two-level graph attention auto-encoder, to integrate these data to uncover cross-modality regulatory interactions and improve spatial clustering.

KtrAB complex is essential in K⁺ uptake for bacteria. Here authors illustrate the synergism of ATP and Na⁺ in activating K+ uptake of KtrAB from Bacillus subtilis and its gating mechanism, which may also shed light on other Na⁺-activated K⁺ channels.

SUMO modification regulates protein function, with SENP enzymes controlling SUMO removal. Here, the authors present crystal structures of SENP5 bound to SUMO1 and SUMO2, revealing how structural features drive its preference for SUMO2 and offering insights into SUMOylation regulation.

Here, the authors develop a self-amplifying RNA encoding the SARS-CoV-2 spike protein encapsulated within a lipid nanoparticle as a vaccine candidate and show induction of neutralization antibody titers in mice that are comparable to titers in convalescent sera of patients.

A combination of adenovirus-vectored and subunit protein intranasal vaccine enhances immune response against SARS-CoV-2 variants in animal models and humans.

Early detection and accurate diagnosis of focal liver lesions are crucial for effective treatment and prognosis. Here, the authors present a fully automated diagnostic system that leverages multi-phase CT scans and clinical features, for diagnosing liver lesions.

Here the authors show how the DNA-sensing cGAS–STING pathway activates NF-κB and inflammatory gene expression with delayed kinetics via post-Golgi endolysosomal signaling.

An antibody, IY-2A, identified from a panel of class-4 SARS-CoV-2-neutralizing antibodies isolated from convalescent and vaccinated individuals, targets and induces partial unfolding of a conserved epitope within the RBD. IY-2A retains activity against BA.4/5 subvariants and neutralizes diverse sarbecoviruses.

The authors obtain tetragonal La3Ni2O6.92 single crystals at ambient pressure by post-annealing in a high-oxygen-pressure environment. Experimental results on these samples suggest that the tetragonal structure is not a prerequisite for achieving superconductivity in La3Ni2O7.

Immune checkpoint blockade therapy for cancer is promising, but delivery optimization is desirable. Here the authors generate cancer-targeting liposomes with anti-PD-L1 and doxorubicin for protease-mediated intratumoral release of both components, and see effective tumor control in mouse models, thereby hinting potential clinical translation.

This study identifies a regulatory module in which UBC32 and SC35 regulate bZIP49 splicing to maintain potassium balance, revealing how poplar adapts to salt stress and providing new insights into plant responses to soil salinity.

Ma et al. show that acetylation of PRDM16 via acetyl-CoA, derived from branched-chain keto acids, impairs PRDM16–PPAR interaction, thus linking the BCAA–BCKA axis to the regulation of white adipose tissue browning.

Dual-topology membrane proteins consist of subunits that have identical sequence but reside in the membrane in two inverted orientations. Here, Woodall et al.find that the dual topology of the transporter EmrE is largely achieved by initial insertion in both topologies rather than major rearrangements after insertion.

Magnesium chelatase catalyses Mg insertion into the centre of protoporphyrin IX during chlorophyll synthesis. The crystal structure of magnesium chelatase from the cyanobacterium Synechocystis shows an active site deeply buried within protein lined with evolutionary conserved residues.

Employing a candidate gene approach, Mancina et al. identify a genetic variant of the Pleckstrin and Sec7 domain-containing 3 (PSD3) gene that reduces susceptibility to fatty liver disease. Functional studies in vitro and in vivo demonstrate that targeting PSD3 protects against fatty liver disease.

Here, using lung epithelial cells and ex vivo tissue explants, the authors show that, in addition to ACE2, host heparan sulfate is directly involved in SARS-CoV-2 attachment and entry and provide data suggesting that host sialic acids may act as viral restriction factor in lung tissues.

Here the authors report the isolation and structural and biological characterization of a humanized angiotensin-converting enzyme 2 (ACE2)-blocking antibody, which exterts potent inhibitory activity against SARS-CoV and circulating global SARS-CoV-2 lineages both in vitro and in hACE2 mouse model.

The small heat-shock protein HSP27 occurs predominantly in oligomeric forms, which makes its structural characterisation challenging. Here the authors employ CPMG and high-pressure NMR with native mass spectrometry and biophysical assays to show that the active monomeric form of HSP27 is substantially disordered and highly chaperone-active.

Vaccines for SARS-CoV-2 are needed to fight the pandemic. Here the authors show immunogenicity of an adjuvanted subunit vaccine, SARS-CoV-2 spike protein trimerized with trimer-tag technology, in small animal models and protection from SARS-CoV-2 challenge in non-human primates.

Neural mechanisms underlying sex-dependent conditioned pain hypersensitivity are not fully understood. Here authors found that a neural circuit from the medial preoptic area to the ventrolateral periaqueductal gray mediates context-dependent pain hypersensitivity in male, but not female, mice, which requires activation of testosterone signaling.

Ligand discovery against membrane proteins has been a major challenge, mainly due to the peculiar nature of their natural habitat. Here, the authors designed a new chemical proteomic probe that targets the lysines exposed on the cell surface and developed a chemical proteomic strategy for global analysis of surface functionality in living cells.

Stress-induced instability of perovskite layers is a critical hurdle for commercialization of perovskite solar cells. Here, the authors introduce a long-alkyl-chain anionic surfactant additive to chemically ameliorate crystallization kinetics and demonstrate devices with long operational stability.

The increase in detoxification gene expression is a common transcriptome marker for longevity interventions. Here, the authors show that nomilin extends lifespan and healthspan in animals through activation of PXR regulated detoxification functions.

Cross-phase manipulation holds potential for applications in synthetic biology and drug delivery. Here, authors present an acoustofluidic platform that enables rapid embedding of microparticles from an oil phase into aqueous droplets, offering an effective tool for studying cellular multiphase interactions and related phenomena.

The underlying mechanisms for drug resistance in Acute Myeloid Leukemia (AML) are not completely understood. Here, the authors show that succinate accumulation, associated with succinate dehydrogenase deficiency, impairs the activity of the ubiquitin-conjugating enzyme UBC12 leading to tumor-promoting protein accumulation and drug resistance in AML.

Early intermittent feeding of mice with a high-cholesterol Western-type diet accelerates atherosclerosis compared with late continuous exposure to the Western-type diet, despite similar cumulative circulating LDL-C levels.

The role of vascular plasticity in brain function remains poorly understood. Here, the authors demonstrate that a significant portion of blood vessels in the adult brain periodically occlude and regress, a process that is associated with a reduction in neuronal activity.

Shi and colleagues present a spatial microfluidic holographic integrated platform for label-free, high-dimensional analysis of cancer heterogeneity, achieving very high accuracy in classifying cancer types, breast cancer subtypes, and blood cells.

Disruption of dendritic cell (DC) interstitial motility in the tumour microenvironment promotes immune evasion, and enhancement of DC interstitial motility offers a route for DC-centric immunotherapy.

Previous work has shown that helical domain walls can form between states of different spin-polarization during a ferromagnetic spin transition in the fractional quantum Hall regime. Here, the authors study the transport through a single helical domain wall and find strong deviations from a simplified theory of weakly interacting edge channels.

A facile and cost-effective synthesis of MXenes is not yet available. Here, the authors propose a one-pot molten salt-based method of MXenes synthesis from elemental precursors in an air atmosphere. Li-ion storage properties of the MXenes are also reported and discussed.