Search

High-throughput chemical ligand discovery is challenged by false positives. Here, authors introduce a scalable enantioselective affinity-selection mass spectrometry approach for proteome-wide ligand discovery with high sensitivity and selectivity

This phage-assisted continuous evolution protocol enables directed protein evolution. It includes preparation of selection phage and host cells, assembly of a continuous flow apparatus and performance and analysis of evolution experiments.

Little Red Dots (LRDs) are a high-redshift galaxy population with unclear nature. Here, authors show CANUCS-LRD-z8.6, a spectroscopically confirmed LRD, hosting an active galactic nucleus, and its properties provide insights for early black hole and galaxy formation.

It is unclear whether shifts in the geographic range of species can keep pace with ongoing climate change. This analysis of 406 species of North American birds reveals that mismatches between species’ niches and modern climates are increasing even for highly mobile species.

Methods used to date a network of marine sediment cores reveal that rapid retreat of the Ross Ice Shelf was contemporaneous with the lowering of nearby outlet glaciers, implicating warm ocean waters as a driver of Antarctic deglaciation.

Using samples and data from the CALERIE study, a human randomized controlled trial of long-term caloric restriction, the authors find that the intervention slows DunedinPACE, a DNA methylation measure of the pace of aging.

ENTRAP-seq systematically links protein sequence to transcriptional output in a native plant context.

Using quantitative brain imaging, the authors show opposite fMRI BOLD signal to metabolic activity due to variable oxygen extraction across the human cortex. This questions the canonical interpretation of fMRI signal in terms of neuronal activity.

Genome-wide analyses in over one million self-reported cases and controls identify genetic variants associated with stuttering and find genetic correlations with autism, depression and impaired musical rhythm, supporting a potential neurological basis for stuttering.

Here authors show loss of AKAP11, a strong genetic risk factor for bipolar disorder and schizophrenia, disrupts PKA proteostasis and signaling, leading to widespread transcriptomic alterations across the brain, particularly in striatal neurons, as well as altered behavior.

Net-zero policies can put pressure on land use, which can conflict with preserving natural landscapes, cultural sites and agricultural areas. Now a study integrates national energy models with proactive and collaborative planning to design net-zero pathways that conserve natural capital and address diverse concerns.

Candida albicans must adapt to hypoxia. Here, the authors discover the sterol C4-methyl oxidase (SMO) Erg251 is essential for extreme hypoxic growth, as well as virulence and colonization, and that SMO inhibitors have therapeutic potential.

A protocol for cryogenic 3D correlative focused ion beam milling using an integrated fluorescence light microscope and montage cryo-ET for nonadherent and adherent mammalian cells, as well as primary Drosophila melanogaster neurons.

DNA-binding phage-assisted continuous evolution improves the specificity of DNA-binding proteins, as demonstrated with TALENs.

Molybdenum disulfide transistors made with an industry-compatible metal–organic chemical vapour deposition method can exhibit both high on-state and low off-state currents with a channel length of 35 nm and contact length of 30 nm.

A brain-to-voice neuroprosthesis enables a man with amyotrophic lateral sclerosis to synthesize his voice in real time by decoding neural activity, demonstrating the potential of brain–computer interfaces to enable people with paralysis to speak intelligibly and expressively.

Marine spatial planning (MSP) and ecosystem restoration are effective approaches to address marine and coastal biodiversity loss and meet Global Biodiversity Framework targets, but have been applied separately to date. This Perspective outlines how ecoscape restoration and climate-smart MSP can be aligned to deliver reciprocal benefits and accelerate biodiversity recovery.

Temporal integration throughout the human auditory cortex is predominantly locked to absolute time and does not vary with the duration of speech structures such as phonemes or words.

Neurocognitive impairment and premature aging often occur in childhood survivors of cancer. Here, the authors investigate the link between biological markers of age and neurocognitive functioning in survivors of childhood cancer who received either central nervous system (CNS)-targeted or non-CNS-targeted therapy.

Antimicrobial resistance continues to be a critical One Health challenge, despite research and policy progress. Building on the past decade of research, this Perspective provides an integrative roadmap for addressing antimicrobial resistance by leveraging the complexities of human and environment interactions.

Genomic deletions upstream of PITX2 are strongly associated with atrial fibrillation, the most common cardiac arrhythmia. Here, the authors report that ectopic PITX2 expression dosage-dependently disrupts pacemaker cell state and function, and atrial rhythm, linking chromatin conformation of the PITX2 locus to both sinus node dysfunction and atrial fibrillation.

The slow maturation of human neurons is regulated by epigenetic modification in nascent neurons, mediated by EZH2, EHMT1, EHMT2 and DOT1L.

Plant community responses to climate change tend to be lagged in forests, but could be faster in grasslands. Here, the authors integrate long-term experimental data with >1 million occurrence records for >300 species, finding grassland community shifts towards species associated with warmer and drier conditions at a pace that aligns with that of climate change.

Population epigenetics leverages methylation profile scores (MPSs) to aggregate information across multiple DNA methylation sites. In this Comment, the authors advocate for MPSs to be trained in early-life contexts, anchored in longitudinal developmental data and evaluated using harmonized standards.

Phage-assisted continuous evolution of Cas9 variants with broad PAM compatibility and high DNA specificity that can be used for transcriptional activation, gene disruption and base editing.

An international team of scientists recovered fossil coral reef samples from the shelf edge of the Great Barrier Reef to reconstruct past reef development and rapid relative sea level rise, including constraints on Meltwater Pulse 1B (11.45–11.1 ka).

Accurately measuring dietary intake has long been a challenge in nutrition research. Integrating emerging tools with multisampling strategies and a dietary assessment methodology aligned with the research aims enables a more objective and comprehensive evaluation of dietary behaviours—and a deeper understanding of diets’ impact on human and planetary health.

Increased river incision and landscape erosion can be attributed to late Cenozoic cooling/changes in hydroclimate, according to cosmogenic isotope and luminescence ages of a sequence of bedrock terraces in the Yukon River basin.

Targeted nanoparticle delivery to sites of interest is important for targeted therapeutics. Here, the authors improve the targeting efficiency of antibodies on nanoparticles using a monobody adapter to correctly orientate the antibody to preserve targeting function and demonstrate application in targeting the vascular endothelium of human kidneys.

Khetarpal et al. show that the metabolic regulator PGC-1α is essential in heart muscle cells for exercise-driven cardiac growth, and that suppression of the stress-induced myokine GDF15 is required to enable cardiomyocyte adaptations to training.

Bourdon et al. demonstrate the possibility to ectopically synthesize callose, a polymer restricted to primary cell walls, into Arabidopsis and aspen secondary cell walls to manipulate their ultrastructure and ultimately reduce their recalcitrance.

Antimatter remains an enigma – its absence in the universe is hitherto unexplained. Here, the authors report a breakthrough in the ability to study antihydrogen atoms to test fundamental symmetries. Antihydrogen atoms can now be accumulated at CERN at the previously unattainable rate of 2000 per hour.

Intracellular Salmonella enterica uses a type III secretion system to secrete more than 30 effector proteins into host cells. Here, Newson et al. generate six mutant strains deficient in different sets of effectors, thus providing insights on the interdependency or functional redundancy between effectors in vivo.

How did eukaryotic cells with complex architecture evolve from simpler prokaryotic cells? DNA analyses offer possible answers.

Several mechanisms are in place to ensure the accurate and timely replication of the genome as cells progress through S-phase. Here, the authors show that Mus81, an endonuclease involved in the response to DNA damage during replicative stress, also regulates the rate of DNA replication during normal growth.

Analysis of the sub-seasonal patterns of river migration reveals that permafrost reduces erosion rates and suggests that full permafrost thaw may lead to a 30–100% increase in the migration rates of Arctic rivers.

Mass spectrometry fragmentation patterns define analytical barcodes for the rapid, quantitative analysis of high-throughput chemical synthesis experiments.

An observationally calibrated ice sheet–shelf model suggests that global warming of 3 °C will trigger rapid Antarctic ice loss, contributing about 0.5 cm per year of sea-level rise by 2100.

It may have taken only a decade to repeatedly destabilize the Antarctic Ice Sheet after the last Ice Age as shown by a new data-model study. The ice sheet lost ice for centuries each time before it abruptly re-stabilized again.

An analysis of coral reefs in the tropical western Atlantic suggests that nearly all will be eroding by 2100 if global warming exceeds 2 °C, which will worsen the effects of sea-level rise.