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Burns et al. introduce an unrolled, physics-informed machine learning method that speeds up blind structured illumination microscopy by orders of magnitude while preserving generalizability, enabling real-time superresolution imaging in live cells.

Laser flash melting experiments have rendered cryo-EM fast enough to observe the microsecond motions of proteins. The authors extend the observation window of this emerging technique tenfold, to hundreds of microseconds, by sealing cryo-EM samples in ultrathin liquid cells.

Here they develop PLOTTED, a novel imaging-based method for extracting chromatin conformation information from 3D data. This method provides visualization tools to assess spatial differences, illustrating the interplay between chromatin conformation and gene expression.

The authors introduce a compact, fast and high-resolution quantitative phase imaging system which integrates nanophotonic metasurfaces with artificial intelligence (AI)-driven algorithms. They demonstrate nanoscale resolution better than 840 nm at a 74 Hz frame rate, all within a single thin optical layer.

Stepp and colleagues present hybrid-EDA, an event-driven acquisition (EDA) that enables gentle investigation of rare mitochondrial events. This approach combines continuous, low-phototoxicity phase-contrast surveillance with event-triggered fluorescence imaging, powered by dynamics-aware machine-learning event detection.

The authors prepare single-domain SrAl_11-δTiO₁₉ ferroelectric thin films with a magnetoplumbite structure through a solid-state reaction. This thin film not only exhibits high remnant polarization but also demonstrates excellent polarization retention characteristics.

Fluorescence microscopy during CryoFIB milling produces an interferogram that can be used to direct lamella production to labeled structures with accuracy beyond the axial diffraction limit. The approach relies only on real-time feedback from the structure, requiring no image registration.

This PrimeView highlights the key steps for reconstructing a super-resolution image from single-molecule localization data.

The authors developed a specialized objective lens that corrects GRIN lens aberrations, enabling in vivo, large–field-of-view, two-photon volumetric calcium imaging of more than 1,000 neurons deep within mouse brains.

Each valley of the mini-Brillouin zone ("mini valley") of twisted bilayer graphene (TBG) contains two Dirac cones that hybridize to form flat bands. Theory predicts that these two Dirac cones have the same chirality, leading to topological obstruction. Here, the authors confirm this prediction experimentally.

A majority methylammonium and iodine edge termination is observed by electron ptychography in the perovskite methylammonium lead iodide, and the stability of its edges and internal defects depends on the concentration and type of vacancies present.

Imaging neural activity across large volumes at subcellular resolution in freely behaving animals remains challenging. Here, a miniature Bessel-beam two-photon microscope enables volumetric calcium imaging of 1,000+ neurons in freely moving mice.

Tau phosphorylation was found to hinder the formation and protective functionality of tau envelopes against microtubule-severing enzymes, providing a potential explanation for microtubule destabilization observed in neuropathology.

Atomic force microscopy is used to investigate the adsorption and organization of ions on charged surfaces. Trivalent ions adopt complex networks, clusters and layers associated with overcharging, whereas divalent ions follow classical predictions.

Screening of 16 capsaicin chemical derivatives leads to the discovery of HMBB, the first mitochondrial inhibitor of the cell cycle protein AURKA in cellular models of breast cancer

Array microscope with mirrored well plates enables automated, scalable 3D behavioral analysis of 48 zebrafish larvae in parallel, revealing complex kinematics and phenotypes missed by conventional 2D methods.

Cardiac ryanodine receptors (RyR2) are critical for heart contraction. Here, the authors use 3D MINFLUX microscopy to image receptor subunits and RyR2 orientation with nanometre resolution, thereby providing a molecular view of the organisation and clustering of these cardiac muscle receptors.

Strain can lead to emergent quantum states. Here, the authors identify a strain induced topological crystalline insulator phase in bilayer SnTe on bulk 2H-NbSe₂.

Volumetric Localization Microscopy (VLM) integrates light-field imaging with deep learning for high-fidelity 3D single-molecule imaging. Trained on system-aware PSFs, VLM offers simple, efficient, low-toxicity 3D imaging for biomedical research.

FACED 2.0 builds on and expands the capabilities of the free-space angular-chirp-enhanced delay microscopy approach. Its high speed, large field of view and volumetric coverage enable two-photon voltage imaging of hundreds of neurons or calcium imaging of thousands of neurons in the mouse or zebrafish brain.

Electron tomography enables high-resolution 3D imaging but is limited by radiation sensitivity. Here, the authors demonstrate a phase retrieval method using multiple tilted HRTEM focal series, achieving 1.6 Å resolution with improved dose efficiency and elemental sensitivity.

Different agonists produce equilibria of at least four distinct active states of the G-protein-bound M2 muscarinic acetylcholine receptor, each with a different ability to activate G proteins.

The Evaluation and Enhancement (EVEN) method optimizes automatically the quality of multichannel microscopy images affected by uneven illumination, enabling accurate visual interpretation and image analysis in challenging imaging scenarios.

Haspels et al. developed a high-throughput assay facilitated by automatic spheroid segmentation using deep learning. Measured differences in treatment response between cisplatin-sensitive and resistant tumors faithfully correspond with expected in vivo responses and the assay is able to discriminate between olaparib-sensitive and resistant tumors.

The authors present quantitative bidirectional light-scattering imaging that simultaneously captures microscale and nanoscale cellular structures, achieving a ~ 14-fold broader dynamic range than standard quantitative phase imaging.

A scanning electron microscope operated at 20 keV with distortion corrected ptychography achieves sub-ångström resolution, thus offering a compact and lower-cost alternative TEM imaging method that is well-suited to 2D materials and small proteins.

Smart microscopy is an emerging technology which integrates real-time analysis with adaptive acquisition to enhance imaging efficiency. Here the authors introduce “outcome-driven microscopy,” an approach that uses optogenetics and real-time feedback to control cell behaviour and protein dynamics.

SmartEM is a ‘smart’ pipeline for electron microscopy-based data acquisition for connectomics. In order to efficiently image large datasets, the approach involves imaging at short pixel dwell times and identifying problematic regions that are then imaged with longer dwell times and therefore higher quality.

The authors develop a high-speed remote focusing method for volumetric voltage imaging enabling imaging at 500 volumes/s. This is combined with light sheet microscopy to record data from >100 spontaneously active neurons in parallel.

This study shows how SARS-CoV-2 fusion peptides assemble and reshape membranes, revealing cooperative mechanisms that lower barriers to viral entry and offering principles for designing antiviral and membrane-responsive nanomaterials.

Authors present a method for providing fast and robust drift correction in single molecule localization microscopy, by pairing the nearest molecules in data segments and calculating their displacements within a small search radius.

AXIS-SIM, a new method of superresolution microscopy, was developed to achieve nearly uniform resolution in all directions with a simple reflector. The advancement allows scientists to observe living cells in unprecedented detail and track dynamics

The authors present the adaptive optics system MD-FSS, which allows high-resolution brain imaging in awake mice with rapid aberrations correction against motion. It reveals differences in brain function between awake and anesthetized states, improving our understanding of neurovascular dynamics.

LSFM imaging revealed 3D islet maps: GADA+ cases mirrored controls, while short-duration T1D showed loss of small INS + GCG– islets but preserved larger mixed INS + GCG+ ones, highlighting islet size- and composition-dependent vulnerability

Auvray and colleagues present a covalent fluorescent probe targeting tubulin, a key cytoskeletal protein. The probe is cell-permeable, fluorogenic, and STED-compatible, enabling clear, minimally-invasive imaging of dynamic processes in live and fixed cells.

The authors propose Analytic Fourier Ptychotomography, for analytically reconstructing aberration-free, complex-valued 3D refractive index distributions without iterative optimization or axial scanning. The method has potential applications in biology, microbial ecology, and clinical science.

Galland et al. present soSMARt, a method for in-depth single molecule localisation microscopy using microfabricated devices, which enables single-objective light-sheet microscopy, adaptive optics correction, real-time registration, and axially extended volume reconstruction with nanometer precision.

Dysregulation of blood vessel size can cause vascular malformations. Here, the authors demonstrate that endothelial cells generate circumferential actomyosin cables to control the size of cells and vessels.

In this work, the authors show that the essential Mycoplasma pneumoniae protein P116 enables cholesterol acquisition from lipoproteins and various cell types. An antibody against its C-terminal domain inhibits lipid acquisition, growth, and plaque binding, linking M. pneumoniae to atherosclerotic lipid-rich tissue.

High-resolution data are crucial for accurate structural modeling. Here, authors enhance MicroED data quality using energy filtering, achieving sub-atomic resolution protein data and uncovering diffuse scattering, offering detailed insights into protein structure and function.

Detecting proton arrangements and quantum behavior at the atomic level is a challenge. Here, the authors use atomic force spectroscopy to reveal symmetry breaking in the proton arrangement in cyclic hydrogen bonds caused by nuclear quantum effects.

ROSE-3D is a single-molecule localization microscopy approach that achieves high isotropic resolution via interferometric localization. The approach is capable of whole-cell and multicolor imaging.

FHIRM-TPM 3.0 is a miniature microscope for multicolor two-photon imaging in freely moving mice. In addition to the multicolor imaging abilities achieved with the help of a specially designed optical fiber, the microscope is also compatible with multiple lenses for a choice of field of view and resolution.

By harnessing the eye’s own red-eye glow as back-lighting, researchers delivered sharp, 2 mm-wide views of live cornea and lens cells, unmasking Fuchs dystrophy and enabling low-cost, precise eye diagnostics.

The authors use multi-slice ptychography to reconstruct depth-resolved phase contrast images of a non-superconducting infinite-layer nickelate superlattice, 8NdNiO₂/2SrTiO₃. The results highlight the presence of disordered residual oxygen and suggest the formation of local domains with varying degrees of oxygenation.

Computer algorithms for contrast and resolution enhancement of microscopic images can become derailed. The authors here provide proof is offered that the Richardson-Lucy algorithm turns out to amplify noise in the initial data, wrecking the final outcome.